摘要
磷脂酶是在生物体内存在的可以水解甘油磷脂的一类酶,其中主要包括磷脂酶A1、A2、B、C和D,它们特异地作用于磷脂分子内部的各个酯键,形成不同的产物,被广泛用于甘油磷脂的改造。依靠传统手段从动物脏器中提取的磷脂酶已经不能满足目前生产的需求,而微生物来源磷脂酶的筛选及其基因工程发展迅速,显著拓宽了磷脂酶的来源,同时又有效地改善了磷脂酶的性质。本文综述了近年磷脂酶高产微生物菌株的选育、磷脂酶的分子改造以及修饰方面取得的进展。
The phospholipases,including phospholipase A1,A2,B,C and D,that are a complex and crucially important group of enzymes that exit in organism and can specifically hydrolyze the intramolecular ester bond of p-hosphoglycerides releasing different products. Thus,phospholipases are widely used in phosphoglycerides transformation. The extraction of phospholipases from animal organs by the traditional means cannot meet the current industry requirements, however,the study of microbial source phospholipases and the rapid development of gene engineering not only broaden the sources of them but also improve their properties effectively. The paper reviewed the progress in the scieening of the high yield strain and phospholipases molecular modification and transformation.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第4期393-396,共4页
Science and Technology of Food Industry
基金
十二五国家科技支撑计划项目(2011BAD02B02)
2012年基本科研-青年基金(常明)(1022050205124000)
关键词
磷脂酶
菌种筛选
固定化酶
化学修饰
基因工程
phospholipase
strains screening
immobilized enzyme
chemical modification
genetic engineering
