摘要
目的:观察芪术颗粒对阿霉素肾病大鼠肝功能的影响。方法:SD大鼠随机分为6组:空白组,模型组,泼尼松(10 mg·kg-1)组和芪术颗粒(按生药量计,5,10,20 g·kg-1)剂量组;除空白组外,其他各组采用阿霉素静脉注射的方法制备大鼠阿霉素肾病模型;连续给药4周,进行血清蛋白、血脂、肝、肾功能的检测以及HE染色病理观察和免疫组化分析肾脏纤维连接蛋白(FN)表达。结果:与空白组比较,模型组动物总胆固醇(TCH)和甘油三酯(TG)水平均明显升高(P<0.01),血清白蛋白以及肝、肾功能下降,肝、肾组织病理形态明显异常,肾脏FN表达明显升高(P<0.01);与模型组比较,芪术颗粒可明显降低模型动物血清TCH和TG水平(P<0.05,P<0.01),提高血清白蛋白水平,改善肝、肾功能,减轻肝、肾的组织病理性损伤,同时降低肾脏FN表达。结论:芪术颗粒长期给药对阿霉素肾病大鼠肝功能具有明显的保护作用。
Objective： To study the effect of Qizhu granule on liver function in rats with adriamycin nephropathy. Method： SD rats were randomly divided to six groups： blank group,model group,prednison group10 mg·kg- 1and Qizhu granule groups with the dosage of 5,10,20 g·kg- 1. All animals,except for blank group,had been intravenously injected with drugs to establish the rat model with adriamycin nephropathy. Four weeks later,the serum albumin and lipid levels,liver and kidney functions were measured at the end of the study. The pathological analysis of hepatic and kidney tissues was performed by hematoxylin and easin（ HE） staining. The expression of fibronectin（ FN） was also measured by immunohistochemical staining. Result： Compared with blank group,the total cholesterol（ TCH） and triglycerides（ TG） levels in serum and the expression of FN in kidney were significantly increased in model group after the 4-week study（ P〈0. 01）,but the serum albumin levels,liver and kidney functions were significantly decreased（ P〈0. 01）. Meanwhile,abnormal pathological changes of hepatic and kidney tissues could be observed by HE staining. Compared with model group,Qizhu granule could significantly decrease the levels of TCH and TG in serum and FN expression in kidney（ P〈0. 05,P〈0. 01）,increase the serum albumin levels,improve liver and kidney functions and attenuate pathological changes of hepatic and kidney tissues. Conclusion： Qizhu granule can mitigate hypohepatia in rats with adriamycin nephropathy by long-term administration.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第22期158-162,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家“重大新药创制”科技重大专项(2012ZX09103201-040)
关键词
芪术颗粒
肝功能
阿霉素肾病
Qizhu granule
liver function
adriamycin nephropathy
