摘要
目的:研究炙黄芪对阿霉素所致小鼠蛋白尿的的防治效果。方法:将Balb/c雄性小鼠随机分为4组:正常组(15只)、预防组(15只)、模型组(15只)、防治组(15只)。预防组、模型组和防治组尾静脉注射阿霉素(10.5 mg/kg)造模。预防组和防治组小鼠造模前灌胃炙黄芪21天,造模后防治组继续灌胃炙黄芪28天,其余各组灌胃等量生理盐水。第2周、第4周末,检测24 h蛋白尿含量;实验结束时采血测定血液生化指标,光镜观察肾组织形态变化。结果:(1)造模后2、4周末,模型组小鼠的尿蛋白总量均高于同期正常组小鼠,预防组(P<0.05)和防治组小鼠的尿蛋白均低于模型组,以防治组(P<0.01)效果最为显著。(2)造模28天,与正常组比较,模型组小鼠血肌酐显著升高(P<0.05),血尿素氮和胆固醇无显著性差异;与模型组相比,预防组和防治组均可降低血肌酐(P<0.05)。(3)模型组小鼠肾小管上皮细胞内可见脂滴,防治组和预防组小鼠脂肪变性的小管细胞少于模型组。结论:炙黄芪预防性给药可减轻阿霉素对肾脏的损伤,表现为降低蛋白尿、改善肾功能;防治效果优于预防效果。
Objective：To investigate the preventive function of Radix Astragali on proteinuria induced by adriamycin（ADR）in mice.Methods：The MCN model mice established by a single intravenous injection of Adriamycin were divided into the model group,Radix Astragali preventive group,Radix Astragali preventive and treatment group,a in additional normal control group.The effect of preventive and treatment on renal function,hemorrheologic parameters and rena1 tissue morphology were observed.Results：After treatment,the parameters of total proteinuria in 24 h and serum creatinine（SCr）between the intervention group and the model group were significantly different respectively,morphological observation also showed the pathological changes in the intervention group were milder than those in the model group.Conclusion：Prophylactic of Radix Astragali might protect mice kidney against injury induced by ADR via decreasing proteinuria.
出处
《中华中医药学刊》
CAS
2013年第3期504-506,I0004,共4页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(81173406)
上海市大学生创新活动计划(SHUTCM CXHDZ(2010)03)
上海市教育委员会重点学科(第5期)项目(J50301)
上海高校创新团队建设项目
关键词
炙黄芪
阿霉素
蛋白尿
小鼠
Radix Astragali
adriamycin
proteinuria
mice
