摘要
目的探讨结核感染T细胞检测阳性患儿外周血T淋巴细胞亚群的变化。方法采用酶联斑点免疫法检测64例结核感染患儿结核特异A抗原和B抗原,并采用流式细胞仪检测其外周血T淋巴细胞亚群比例,同时测定40例健康儿童外周血T淋巴细胞亚群比例做对照。结果与对照组比较,T-SPOT.TB阳性组患儿外周血CD3+、CD4+、CD4+/CD8+细胞表达率降低,差异有统计学意义(P<0.01);NK细胞细胞表达率升高,差异有统计学意义(P<0.05);CD8+、CD19+细胞表达率差异无统计学意义(P>0.05)。结论儿童患结核后细胞免疫功能受到抑制,细胞免疫在调节中起着重要作用。
Objective To investigate the the value of T cell in the detection of T lymphocyte subsets changes of peripheral blood in children with tuberculosis infections. Methods Enzyme- linked immunospot assay was applied to detect the antigens A and B,and the flow cytometry was used to determine the T cells subpopulations in 64 children with tuberculosis infections,meanwhile,the T cells subpopulations in 40 healthy donors were also detected by using the flow cytometry for comparison. Results Compared with the control group,the T cell detection of tuberculosis showed that the expression rate of CD3＋,CD4＋,CD4＋/ CD8＋decreased in the peripheral blood of children in the positive T- SPOT. TB group,and the difference had statistical significance（ P〈 0. 01）; the expression rate of NK cell increased,and the difference had statistical significance（ P〈 0. 05）; the expression rate differences of CD8＋and CD19＋had no statistical significance（ P〈 0. 05）. Conclusion The cellular immune function was significantly inhibited after children suffering from tuberculosis,and it played an important role in the regulation.
出处
《中国卫生检验杂志》
CAS
2015年第21期3669-3670,共2页
Chinese Journal of Health Laboratory Technology
关键词
儿童细胞免疫功能
淋巴细胞亚群
酶联斑点免疫法
流式细胞术
Cellular immune function in children
Lymphocyte subsets
Enzyme-linked immunospot assay
Flow cytometry
