摘要
目的:探讨脑梗死(CI)患者与其N5,10亚甲基四氢叶酸还原酶(MTHFR)基因多态性的关系。方法:运用聚合酶链反应-限制性内切酶片段长度多态性技术(PCR-RELP)检测脑梗死患者MTHFR基因多态性。结果:MTHFR基因型有3种,即纯合子(C/C)型、杂合子(T/C)型、纯合子(T/T)型。3种基因型在病例组和对照组研究对象间的分布无显著性差异,但T等位基因在病例组和对照组研究对象间的差异有统计学意义。结论:MTHFR基因C677T突变与脑梗死的发生有关,MTHFR基因可能是脑梗死的易感基因之一。
Objective: To explore relationship among patients with cerebral infarction( CI) and their 5,10-methylenetetrahydrofolate reductase( MTHFR) gene polymorphisms. Methods: The polymorphisms of MTHFR gene were determined by using polymerase chain reaction and restriction fragment length polymorphism( PCR-RELP). Results: There were three kinds of genotype: T / T( homozygous mutation),T/C( heterozygous mutation) and C/C( wild-type). There was no significant difference between the two groups for the distributions of the three kinds of genotype,while the difference of T allele was statistically significant between the two groups. Conclusions: C677 T allele mutation of MTHFR gene may be associated with cerebral infarction,and MTHFR gene may be one of the susceptibility genes for cerebral infarction.
出处
《中国民康医学》
2015年第21期60-62,共3页
Medical Journal of Chinese People’s Health
