摘要
目的观察不同浓度高氧对大鼠肺组织NF-κB及IL-8表达的影响,探讨高氧所致肺损伤(HILI)的发生浓度及机制。方法 32只成年雄性SD大鼠随机分为4组,即对照组(吸入空气)、50%O2组、70%O2组和90%O2组,吸氧时间均为96 h。采用RT-PCR法检测肺组织NF-κB p65 m RNA表达水平,采用ELISA法检测支气管肺泡灌洗液(BALF)IL-8含量,测定肺组织湿干重比值(W/D)和BALF中性粒细胞计数,并在光镜下观察各组大鼠肺组织的病理学改变。结果随着吸氧浓度的增加,肺组织NF-κB p65 m RNA表达水平、BALF中IL-8的含量、肺组织W/D比值及BALF中性粒细胞计数均逐渐增加,其中90%O2组增加最明显,与对照组、50%O2组及70%O2组比较差异均有统计学意义(均P<0.05),而对照组、50%O2组和70%O2组之间各项指标比较差异均无统计学意义(均P>0.05)。相关性分析表明,肺组织NF-κB p65 m RNA与BALF中IL-8含量之间呈正相关(r=0.858,P<0.01)。结论吸入氧浓度超过90%且持续96 h以上,即可导致大鼠肺组织损伤,其发生可能与高氧激活肺组织细胞表达NF-κB,上调IL-8表达,导致中性粒细胞聚集和活化而引起的炎症反应有关。
ObjectiveTo explore the concentration and the mechanism of hyperoxia-induced lung injury （HILI） by observing the effects of NF-κB and IL-8 in the lung tissue of the SD rats suffered from exposure to high concentration oxygen of different oxygen concentrations.Methods32 healthy male SD rats were randomly divided into four groups： control group, 50% oxygen concentration group, 70% oxygen concentration group and 90% oxygen concentrationgroup and the inhaled-oxygen time was 96 h. The expression of NF-κB mRNA in lung tissue was detected by reverse transcription-polymerase chain reaction. The contents of IL-8 in bronchoalveolar lavage fluid （BALF） was measured by enzyme linked immunosorbent assay （ELISA）. The lung wet/dry weight ratio （W/D） and the neutrophils count in bronchoalveolar lavage fluid （BALF） were measured. The pathological changes of the lungs were observed under light microscope.ResultsWith the increase in the concentration of inhaled oxygen, the data of W/D of the lung, neutrophils count in BALF, the NF-κB mRNA and the IL-8 contents in BALF were gradually increased, especially in the 90% O2 group which were significantly increased compared to the control group, 50% O2 and 70% O2group （P〈0.05）. Of the indicators above, there were no significant difference existed among control group, 50% O2 and 70% O2 group （P〉0.05）. Correlation analysis showed that NF-κB and IL-8 were positively correlated （r=0.856,P〈0.001）.ConclusionsThe exposure to≥95% O2 for 96 h can lead to lung injury in adult rats with the indicators significantly increasing, which may be associated with the activation of NF-κB. NF-κB when activated can up-regulate the expression of IL-8 in the lung tissue of rats and lead to the inflammatory injury eventually by way of aggregation and activation of neutrophils.
出处
《中华临床医师杂志（电子版）》
CAS
2015年第19期80-83,共4页
Chinese Journal of Clinicians(Electronic Edition)
基金
山西省科技成果推广计划项目(20140710)
