摘要
为提高木醋杆菌(Acetobacter xylinum)发酵苹果渣生产细菌纤维素(Bacterial cellulose,BC)的生产效率,采用单因素及正交实验对纤维素酶水解苹果渣工艺进行优化,同时利用傅里叶红外光谱(FT-IR)和X-射线衍射(XRD)对发酵产物BC的性能和结构进行比较。单因素及响应面实验结果确定苹果渣最优水解工艺:液固比4 m L/g,酶用量16.8 EGU/g,p H5.5,水解温度55℃,水解时间40 h。此条件下,苹果渣水解产生还原糖产率为38.02%。苹果渣水解液发酵产物BC结构性能与基本培养基发酵产物BC基本一致。研究结果表明苹果渣能够作为发酵原料发酵生产BC,且不影响BC性能。
In order to increase the producing efficiency of bacterial cellulose（BC）fermented by Acetobacter xylinum using pomace, single factor and orthogonal experiments were used to optimize the hydrolysis process of pomace. Meanwhile, the properties of BC were compared through fourier transform infrared（FT-IR） and X-ray diffraction（XRD）. According to the results of experiments, the optimal hydrolysis process were determined as follows： liquid-solid ratio 4 mL/g, dosage of celluclast 16.8 EGU/g, pH5.5, hydrolytic temperature 55 ℃,hydrolytic time 40 h. Under these conditions, the productive rate of reducing sugar was 38.02 %. The properties and structures of BC produced with pomace hydrolysate were confirmed to be basically same with BC produced with basic medium, which indicated that the pomace hydrolysate could be used as part of raw materials to ferment BC and would not affect the properties of BC.
出处
《食品研究与开发》
CAS
北大核心
2015年第18期106-110,共5页
Food Research and Development
基金
陕西省科技厅自然基金项目(2012JM2005)
陕西省教育厅科研专项项目(15JK1108)
2013年国家级大学生创新创业训练计划项目(201310708014)
关键词
苹果渣
细菌纤维素
纤维素酶
正交试验
pomace
bacterial cellulose
celluclast
orthogonal experiments
