摘要
目的 分析不同部位参考电极诱发豚鼠听性脑干反应（ABR）的差异。方法健康豚鼠12只（24耳）,分别采用双侧耳廓或双侧乳突作为参考电极,鼻尖部为接地电极,颅顶为记录电极,给予短声刺激,分别观察以耳廓和乳突为参考电极时的ABR波形、反应阈、潜伏期、波间期。结果 两种部位参考电极记录的ABR反应阈,波Ⅰ、Ⅱ、Ⅲ潜伏期,Ⅰ-Ⅲ波间期差异均无统计学意义（P〉0.05）。随着刺激声强减弱,参考电极置于双侧耳廓时,ABR波Ⅱ波形稳定、清晰,振幅下降幅度低于其他各波,最后消失;参考电极置于双侧乳突时,ABR波Ⅲ振幅下降幅度低于除波Ⅳ以外的其他各波,最后消失,同时Ⅱ-Ⅲ、Ⅲ-Ⅳ波间期缩短。在刺激声强下降20-40dB nHL时,两种部位参考电极记录的ABR波Ⅳ振幅增大、潜伏期前移,Ⅲ-Ⅳ波间期缩短,此现象在双侧乳突记录的ABR波形中更显著。结论 同等刺激条件下参考电极置于耳廓记录的ABR波Ⅱ和参考电极置于乳突记录的ABR波Ⅲ波形稳定易辨,两种部位参考电极记录的ABR反应阈、潜伏期、波间期无明显差异;以耳廓为参考电极记录的ABR波Ⅱ可作为豚鼠ABR反应阈的观察指标。
Objective To observe auditory brainstem response evoked at different electrode positions adopting electrode slices.Methods The ABRs of 12 normal guinea pigs（24ears）were recorded by two methods using clicks as stimulation.Active electrode was positioned at the vertex while the ground electrode at the nose.The reference electrode was placed on bilateral pinnas or mastoids.Then observe waveforms,response thresholds（RT）,peak latencies（PL）and inter-peak latencies（IPL）of ABR.Results No significant difference were observed in the RT,Ⅰ,Ⅱand Ⅲ PL,Ⅰ-Ⅲ IPL of ABR between the two methods（P〉0.05）.When stimulation was decreasing and with the reference electrode slices placed at bilateral pinnas,waveⅡpeak was stable and discernable of ABR recordings across twenty-four ears of guinea pigs.The amplitude decreased more slowly than any other waves,and disappeared at the last.While reference electrode slices were placed on bilateral mastoids,the amplitude of waveⅢ decreased more slowly than any other waves except wave Ⅳ,and disappeared at lost.Ⅱ- Ⅲ,Ⅲ- Ⅳ IPL became shorter.These two positions both recorded amplitude higher and PL advanced for wave Ⅳ as the stimulation decreased by 20 to 40dB nHL,and Ⅲ-ⅣIPL became shorter.This phenomenon was more obvious when reference electrode was placed at mastoid.Conclusion WaveⅡwas more stable and discernible with reference electrode placed at the bilateral pinnas.Therefore waveⅡrecorded by this way can be a new method to record the ABR threshold of guinea pigs.
出处
《听力学及言语疾病杂志》
CAS
CSCD
北大核心
2015年第6期612-616,共5页
Journal of Audiology and Speech Pathology
基金
白求恩医学科研支持计划--前沿交叉学科创新项目(2013105012)资助
关键词
听性脑干反应
潜伏期
阈值
耳廓
乳突
Auditory brainstem response
Lantency
Peak lantency
Pinna
Mastoid
