摘要
目的:分析不同方式鼓室内给药后豚鼠耳蜗外淋巴液中地塞米松(DEX)代谢动力学特征,为临床选择鼓室内给药方式提供实验依据。方法:采用浸满DEX溶液明胶海绵颗粒置于圆窗龛内和鼓室内灌注两种鼓室内给药方式,采集给药后1～6h豚鼠鼓阶外淋巴液,应用高效液相色谱仪分别检测其中的DEX浓度。结果:相同浓度(10g/L)的DEX溶液经明胶海绵圆窗龛给药后外淋巴液中的DEX浓度1h时最高,DEX生物半衰期t1/2为2.37h。鼓室内灌注给药后外淋巴液中的DEX浓度1h20min时最高,DEX生物半衰期为t1/2为2.26h。除1h20min、1h40min和2h外,两种方式鼓室内给药后外淋巴液中DEX的浓度相比差异均有统计学意义(均P<0.05)。结论:明胶海绵颗粒圆窗龛给药和鼓室内灌注给药两种方式鼓室内给药后DEX均能有效地经圆窗膜渗透进入外淋巴液,大部分时间明胶海绵颗粒圆窗龛给药外淋巴液中的DEX浓度高于鼓室内灌注给药。明胶海绵圆窗龛给药的生物半衰期较长。
Objective:To determine pharmacokinetics profiles of dexamethasone (DEX) in perilymph of guinea pig with different intratympanic administrations,and to provide experimental evidence for clinical choice about administration.Method:Two different topical administrations placing a granule gelfoam soaked with DEX(10 g/L) in the niche of round window of guinea pig and infusing tympanic bulb with DEX solution(10 g/L) were conducted. Perilymph of the scala tympani was sampled 1-6 h after administration.DEX in the samples was assayed with high-performance liquid chromatograghy.Result:The concentrations of DEX in perilymph after placing a granule gelfoam soaked with DEX in the niche of round window were significantly higher than that after intratympanic infusing.The half-life time of DEX after placing a granule gelfoam was longer.Conclusion:DEX could effectively penetrate round window membrane into perilymph after different intratympanic administrations, and the method of placing a granule gelfoam soaked with DEX in the niche of round window is more effective than that of intratympanic infusing.
出处
《临床耳鼻咽喉科杂志》
CSCD
北大核心
2005年第7期307-310,共4页
Journal of Clinical Otorhinolaryngology
基金
上海市科委基金资助项目(No:034119819)
