摘要
为了探讨趋化因子MCP-1及其受体CCR2是否参与了SE初期的病理过程,应用SE的匹罗卡品模型,检测SE 30 min、SE 1 h、SE 2 h和SE 1 d时,MCP-1及其受体CCR2的mRNA在小鼠海马的表达情况.结果显示,MCP-1及其受体CCR2在小鼠海马组成型表达.与对照组相比,SE 1 h时,MCP-1在海马CA1区显著下调(P<0.01);SE 1 d时,CCR2在CA3区显著下调(P<0.05).结果说明,MCP-1及其受体CCR2在成年小鼠海马的活动中具有重要的生理功能;MCP-1/CCR2的下调与SE初期病理过程相关,可能由于下调导致了它们的神经保护作用减弱.
To investigate if chemokine MCP- 1 and its receptor CCR2 participate pathogenesis at the early stage of epilepsy,expression of mRNA of the two genes in mouse hippocampus under SE 30 min,SE 1 h,SE 2 h and SE 1 d was investigated using pilocarpine model of SE. The results showed that MCP- 1 and CCR2 were expressed in normal hippocampus of mouse constitutively. Compared to control group,MCP- 1 was significantly downregulated in CA1 of hippocampus at SE 1 h（ P〈 0. 01） and CCR2 was significantly downregulated in CA3 at SE 1 d（ P〈 0. 05）,which suggests that MCP- 1 and CCR2 might play important roles in hippocampus. The downregulation of MCP- 1 and CCR2 in the hippocampal neurones at the early stage of SE may weaken the neuroprotective mechanisms.
出处
《云南大学学报（自然科学版）》
CAS
CSCD
北大核心
2015年第6期916-921,共6页
Journal of Yunnan University(Natural Sciences Edition)
基金
国家自然科学基金(81160164)
云南省应用基础基金(2008CD117)
