摘要
目的:本研究以常见的22种具有抗肿瘤活性的中药材为对象,对其抗氧化活性进行研究。方法:以DPPH自由基清除率为指标,测定各试样的抗氧化活性;通过FRAP法,Folin-Ciocaheu法以及铝盐显色法,比较其总还原力,总酚含量以及黄酮含量。结果:抗氧化活性大于50%的药材占到了90.09%,其中莪术、虎杖、野生黄芩、白芍、凤尾草、丹参、川断、姜黄等8种药材DPPH的清除作用均超过了90%,显示了较强的抗氧化作用,且野生黄芩的IC50最低(IC50=0.05g/L),抗氧化能力最强,与总还原力的实验结果一致,总酚和黄酮含量也在所有测试品中均为最高,分别为5 240μg/L、83 210μg/L。结论:除了薏苡仁和茯苓,各试样都有较强的抗氧化活性,表明天然抗氧化剂与其抑癌作用具有一定的相关性,但其相互作用机制需要进一步的探讨研究,本研究为从广泛的天然抗肿瘤资源中寻找抗氧化物质及开发抗氧化型抗肿瘤药物提供了实验依据。
Objective： In this study,22 Chinese herbal medicines for anti-cancer were developed for the study of its antioxidant activity. Methods： The antioxidant activities of samples were evaluated by DPPH method; phenols,flavonoids contents and total reducing power were estimated by using the Folin-Ciocalteu reagent,aluminum salt colorimetric method and ferric-reducing antioxidant power assay（ FRAP）. Results： The results confirmed that 90. 09% of the medicine had characterized the antioxidant activity with more than 50%,which included eight kinds of plant materials such as Rhizoma Curcumae,Rhizoma Polygoni Cuspidati,Wild Skullcaps,Radix Paeoniae Alba,Fern,Salviamiltiorrhiza,Radix Dipsaci Asperoidis,Curcuma longa demonstrated better antioxidant activity（ activity 90%）. Wild Skullcaps demonstrated the best antioxidant activity with the lowest IC50 value of 0. 05 g / L,the highest phenolic and flavonoid contents of 5240μg / L and 83210μg / L. Conclusion： Each sample for anti-cancer has a strong antioxidant activity except for the Coix seed and Poria,which confirms natural antioxidant and antitumor effect has a certain correlation,but its mechanism needs further research. In this study,it is possible to find antioxidants and antioxidant-based anticancer drugs develop from broad anti-tumor natural resources providing an experimental basis.
出处
《中医药学报》
CAS
2015年第5期31-35,共5页
Acta Chinese Medicine and Pharmacology
基金
国家自然科学基金(31360379)
