摘要
目的:研究百解胶囊对大鼠肝药酶CYP2C19、CYP2E1活性的影响,探讨其"解药物毒"作用的机制。方法:将大鼠随机分为4组,即空白对照组、百解胶囊组(2.43g生药/kg)、百解胶囊组(0.27g生药/kg)、苯巴比妥钠组(10.8mg/kg),按上述剂量灌胃给药。以甲硝唑为内标,建立HPLC方法检测Cocktail探针药物奥美拉唑和氯唑沙宗在大鼠体内的代谢情况,评价百解胶囊对肝药酶CYP450的影响。结果:与空白对照组比较,百解胶囊组(2.43g生药/kg)对奥美拉唑的清除率(CL/F)明显增强,曲线下面积(AUC)明显减少,其半衰期(t1/2)亦有减少趋势;百解胶囊组(2.43g生药/kg)和百解胶囊组(0.27g生药/kg)对氯唑沙宗的清除率(CL/F)明显增强,曲线下面积(AUC)明显减少,半衰期(t1/2)明显缩短。结论:百解胶囊对大鼠肝药酶CYP2C19、CYP2E1具有诱导作用,可能是其"解药物毒"作用的机制之一。
Objective： Study baijie capsule on cytochrome P450 isozymes CYP2C19,CYP2E1 in rats to explore the mechanism of ＂ Solutions of drug toxicity＂. Methods： The SD rats were randomized diveded into four groups,control group,baijie capsule group（ 0. 27 g / kg）,baijie capsule group（ 2. 43 g / kg）,phenobarbital group（ 10. 8mg / kg）. Oral administration according to the above dose. Control group was given normal saline. Cocktail probe drugs method has been established to determine the two probes of omeprazole,chlorzoxazone and the internal standard was metronidazole to evaluate the effect of CYP450 activity following administration of baijie capsule. Results： Compared with the control group,baijie capsule group（ 2. 43 g / kg） increased the CL / F of omeprazole,reduced the AUC significantly,and t1 /2 was also a decreasing trend. Baijie capsule group（ 2. 43 g / kg） and baijie capsule group（ 0. 27 g / kg） increased the CL / F of chlorzoxazone,reduced the AUC and t1 /2 significantly. Conclusion： Baijie Capsule has an inductive effect on CYP2C19,CYP2E1 in rats,it could be one of mechanisms on ＂ Solutions of drug toxicity＂.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2015年第4期163-166,共4页
Pharmacology and Clinics of Chinese Materia Medica
基金
国家自然科学基金(NO:00360103100)
