摘要
目的考察香附四物汤效应部位体外透皮吸收性质。方法建立UPLC-MS/MS方法测定香附四物汤效应部位(BW)以及透皮接收液中6个主要活性成分(阿魏酸、芍药苷、芍药苷内酯、四氢非洲防己碱、普鲁托品、延胡索乙素)的量;将这6个活性成分按照BW中的比例混合制备活性组分群;采用改良Franz扩散池以及离体大鼠腹部皮肤进行体外透皮吸收实验。结果 UPLC-MS/MS方法专属性良好,各成分均有良好的线性关系(r>0.997)、精密度(RSD≤2.66%)、重复性(RSD≤2.98%)、稳定性(RSD≤3.99%)和准确性[平均加样回收率(95.22±5.48)%^(103.68±2.90)%]。活性组分群和BW中活性成分的累积透过率顺序相同:阿魏酸>四氢非洲防己碱>延胡索乙素>普鲁托品>芍药苷≈芍药内酯苷,且供给液的p H值显著影响活性成分的体外透皮吸收。结论活性组分群与BW体外透皮吸收行为具有一致性,p H值能够改变活性成分的体外透皮吸收行为。
Objective To study the in vitro transdermal absorption properties of active fraction from Xiangfu Siwu Decoction（XSD）. Methods UPLC-MS/MS method was established for the determination of six main active ingredients（ferulic acid, paeoniflorin, albiflorin, tetrahydrocolumbamine, protopine, and tetrahydropalmatine） in active fraction of XSD（BW） and transdermal receiving liquid. The active ingredient group of six active ingredients was prepared according to their proportion in BW. The experiment of in vitro transdermal absorption was performed using vitro-abdominal skin of rats and improved Franz diffusion cell method. Results UPLC-MS/MS method showed perfect specificity and the six ingredients performed well in linear relationship（r 〉0.997）, precision（RSD ≤ 2.66%）, repeatability（RSD ≤ 2.98%）, stability（RSD ≤ 3.99%）, and average recovery rate [（95.22 ± 5.48）%—（103.68 ± 2.90）%]. The cumulative transmittance of the six ingredients displayed the same order between the active ingredient group and BW. The cumulative transmittance in descending order was as follows： ferulic acid tetrahydrocolumbamine tetrahydropalmatine protopine paeoniflorin ≈ albiflorin. Additional, transdermal absorption properties of the six ingredients in vitro were affected by the p H value of supply liquid significantly. Conclusion The in vitro transdermal absorption behavior of the active ingredient group is consistent with BW and p H value can change the behavior of active ingredients in in vitro transdermal absorption.
出处
《中草药》
CAS
CSCD
北大核心
2015年第20期3017-3022,共6页
Chinese Traditional and Herbal Drugs
基金
江苏省高校自然科学研究项目(14KJB360001)
江苏省中药资源产业化过程协同创新中心项目(ZDXMHT-1-15)
江苏省“青蓝工程”
江苏省高校优势学科建设工程资助项目
关键词
香附四物汤
效应部位
活性组分群
体外透皮
UPLC-MS/MS
Xiangfu Siwu Decoction
active fraction
active ingredient group
in vitro transdermal absorption
UPLC-MS/MS
