摘要
目的:建立测定中药复方四物汤中主要成分含量的分析方法,比较四物汤共煎液与单煎混合液中没食子酸、芍药内酯苷、芍药苷、阿魏酸和苯甲酸的含量差异。方法:采用HPLC方法对四物汤共煎液与单煎混合液中的上述5个主要成分进行含量测定。色谱柱为Zorbax SB C_(18)(5μm,250mm×4.6mm)。流动相:0.01%(v/v)磷酸水溶液(A)-乙腈(B)梯度洗脱,洗脱程序为:0-7min,A-B保持95:5不变;7-12min,A-B由95:5线性变化至83:17;12-25min,A-B保持不变;25-27min,A-B由83:17线性变化至80:20;27-35min,A-B保持不变。流速:1.0mL·min^(-1);检测波长:230nm;柱温:25℃,进样量:10μL。结果:这5个化合物在测定的范围内表现出良好的线性(r>0.9995),方法的回收率在94.8%-103.1%之间。四物汤共煎液中没食子酸、芍药内酯苷、芍药苷、阿魏酸的含量均高于单煎混合液,而苯甲酸在四物汤共煎液中的含量低于单煎混合液。结论:四物汤共煎可提高有效成分的溶出,降低有毒成分的含量。
Objective: To develop a method for the determination of gallic acid, albiflorin, paeoniflorin, ferulic acid and benzoic acid in Siwu decoction, and analyze the contents of these five compounds in co-boiling and pooled individual boiling extracts. Methods: An HPLC method was applied with a Zorbox SB C-18(5μm,250mm×4. 6 mm) column by gradient elution using 0. 01%(v/v)phosphoric acid-acetonitrile as the mobile phase(0-7min 95:5,12-25min 83:17,27-35min 80:20). The flow rate was 1mL?min^(-1). The detection was set at UV 230nm. The column temperature was 25℃ ,and the sample injection volume was 10μL. Results: The recovery of the method was in the range of 94. 8%-103. 1%,and all the compounds showed good linearity(r>0. 9995) in a detected concentration range. The contents of these five compounds were different in co-boiling and pooled individual boiling extracts. Conclusion: The contents of active compounds(paeoniflorin and ferulic acid) increase and toxic constituents(benzoic acid) decrease significantly after boiling the individual crude drugs together.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2005年第1期1-4,共4页
Chinese Journal of Pharmaceutical Analysis
基金
北京市科技攻关项目(010210220113)
