摘要
为探讨水稻白化复绿突变体白784的生理特性和相关遗传机制，研究了不同温度处理下白784的表型和生理特性，结果表明，白化表型受温度控制，20、22、24℃低温处理使白784叶片的色素合成不同程度受阻，叶片呈白化表型；与对照相比，白784的叶绿素a、b和类胡萝卜素含量显著下降，20℃处理（10d）白784叶绿素a、叶绿素b较对照分别下降95．18％（P〈0．01）、86．86％（P〈0．01）：22℃处理（14d）白784叶绿素a、叶绿素b、类胡萝卜紊较对照分别下降96．19％（P〈0．01）、93．25％（P〈0．01）、92．76％（P〈0．01）；24℃处理（12d）白784叶绿素a、叶绿素b、类胡萝卜素较对照分别下降86．17％（P〈0．05）、91．67％（P〈0．01）、95．30％（P〈0．01）；26℃处理（12d）白784叶色绿化，叶绿素a、b和类胡萝卜素含量较对照分别下降27．63％、25．70％、27．17％（P〉0．05）：28℃处理（12d）白784叶色与正常无异，叶绿素a、b和类胡萝卜素含量较对照差异不显著；表明24～26℃是白784叶色白化的敏感温度。同时构建F2作图群体（白784×郑州早粳），进行遗传分析和基因定位，结果表明，该突变体温敏白化性状受1对隐性核基因控制；利用424个SSR分子标记将Abt6初步定位在水稻第6号染色体上，位于标记RM5988～RM6071，与标记的遗传距离分别为2．4、9．7cM；进一步扩大F2群体，在F2、F3群体白化单株中寻找目标区段发生重组的个体。利用InDel标记分析目标区段杂合单株的标记基因型，将Abt6定位在InDel标记Y15420～Y15648．标记间物理距离约278kb。
In order to study the physiological characters and relative genetic mechanism of green-reverti- ble albino mutation line Bai 784,the phenotype and physiological characters were studied under different temperature. The results showed that the albino phenotype was controlled by temperature. Under the tem-perature of 20 ℃,22 ℃ ,24 ℃ , pigment synthesis of the mutant seedling was hindered, and the mutant seedling showed albino phenotype. Compared with the control, the content of chlorophyll a,b significantly decreased by 95.18% ,86.86% respectively under 20 ℃for 10 d; the content of chlorophyll a,b and ca- rotenoid significantly decreased by 96. 19% ,93.25% ,92.76% respectively under 22 ℃ for 14 d; the content of chlorophyll a, b and carotenoid decreased by 86. 17% ,91.67% ,95.30% respectively under 24 ℃ for 12 d; the mutation line turned green,and the chlorophyll a,b and carotenoid content decreased by 27.63% ,25.70% ,27. 17% respectively under 26 ℃for 12 d; the mutant leaf color was nearly the same as the control under 28 ℃ for 12 d,and there was no difference in the chlorophyll a,b and carote- noid content between mutant and control,indicating that 24 -26 ℃ was the sensitive temperature for albi- no of leaf. The gene fine mapping was doue with F2 mapping population of Bai 784 x Zhengzhouzaojing, and the results showed that the albino phenotype was controlled by a single nuclear recessive gene （Abt6） ,which was mapped on chromosome 6 between SSR markers RM5998 and RM6071 with a genetic distance of 2.4 cM and 9.7 eM respectively. Screening F2 and F3 progenies for lines whose marker geno- type was heterozygous in target interval,with which and some new InDel markers the target gene was de- limited to a 278 kb interval between Y15420 and Y15648.
出处
《河南农业科学》
CSCD
北大核心
2015年第10期17-23,共7页
Journal of Henan Agricultural Sciences
基金
河南省农业科学院发展专项资金项目
河南省重大科技专项(121100110200)
河南省水稻产业技术创新团队首席专家项目(S2010-04)
河南农业科学院农业科技创新项目(201315619)
关键词
水稻
白化复绿
白784
精细定位
rice
green-revertible albino
Bai 784
fine mapping
