摘要
用￣（６０）Ｃｏγ射线诱发的温敏核不育水稻叶色突变体中，发现有４个突变体的叶色表达与温度有关。突变体Ｗ＿４、Ｗ＿１１在高温（３０～３５℃）下叶片呈白色，在低温（２０～２５℃）下表现为黄色或浅黄色。３５℃时叶绿素（ａ＋ｂ）含量分别为０．０２１９和０．０５３６ｍｇ／ｇ鲜重，而在２５℃时分别上升到０．２４１０和０．３４３１ｍｇ／ｇ鲜重，增长幅度达１０倍左右。相反，突变体Ｗ＿１７和Ｗ＿２５在低温下表现为白色，高温下转为浅绿色。在２５℃时叶绿素含量分别只有０．０８１３和０．０１７２ｍｇ／ｇ鲜重，在３５℃时分别上升到１．０５７０和１．１３６７ｍｇ／ｇ鲜重，增长幅度高达９０倍以上。在此温度范围内，对照２１７７Ｓ的叶绿素含量在同一数量级内变化，在生长过程中，上述突变体的叶片均可转换成黄绿色或绿色，并开花结实，在２５℃下生长到３．５叶期时，Ｗ＿４和Ｗ＿１１的叶绿素含量已接近２１７７Ｓ的水平，分别达对照的８６．６％和８１．１％。但Ｗ＿２５在２０℃及以下温度生长时，叶片始终呈白色，不发生转换，从而表现出条件致死的特性。
Studies on the leaf color appearance of 4 chlorophyll-deficient mutation lines both infield and in phytotron were carried out.The mutation lines were induced by 60 Co gammarays,and showed that white or yellow leaves at seedling stage were quite different from theirparent 2177S,a thermal sensitive genic male sterile line and any other rice materials.Thetemperature had great influence on the expression of leaf color at seedling stage in the muta-tion lines. The leaf color was white at 30～35℃ for the lines W4 and W11.The chlorophyllcontent of 1.5-leaf-age seedlings was 0.0219 and 0.0536mg/g FW respectively for W4 andW11 at 35℃,When the temperature dropped to 20～25℃,the seedlings showed yellow or yel-lowish and the chlorophyll content reached to 0.2410 and 0.3431mg/gFW at 25℃,respec-tively.However,the responses to temperature for W17 and W25 were just the opposite. Theywere white at 20～25 ℃,but appeared greenish at 30～35℃, The chlorophyll content in-creased from 0.0813 and 0.0172mg/g FW at 25℃ to 1.0570 and 1.1367mg/g FW at 35℃ forthe lines W17 and W25,respectively.The parent line 2177S showed normal green and thechlorophyll content was between 2.108 and 2.118mg/g FW,The W11 is exception, whichshowed yellow to light green in lifetin1e,and all the mutation lines could convert to normalgreen after the extension of the fourth leaf. The chlorophyll content of 3.5-leaf-age W4 andW17 seedlings grown under 25℃ reached to 2.2190 and 1.993mg/g FW, which was about 86.6%and 81.1%of that of 2177S at the same stage.When grown at the temperature bellow20℃,W25 maintained white and could not changed into green after the 4th leaf extension,and showed a conditional lethal status.
出处
《核农学报》
CAS
CSCD
1996年第1期6-10,共5页
Journal of Nuclear Agricultural Sciences
基金
浙江省"8812"计划资助
关键词
叶色突变
温度
叶绿素含量
水稻
Leaf color mutation,temperature,chlorophyll content, conditional lethal mutation,Oryza sativa L.
