摘要
目的探讨内向整流钾离子通道蛋白J亚单位11号成员(KCNJ11)基因多态性与新疆哈萨克族人群原发性高血压(essential hypertension,EH)的相关性。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测KCNJ11基因多态性位点rs2285676的基因型,其中新疆哈萨克族高血压组(EH组)126例,正常对照组(NT组)126例。应用多因素非条件Logistic回归分析评价新疆哈萨克族高血压相关危险因素。结果 Logistic回归分析显示,KCNJ11-rs2285676位点基因型、性别、体质量、总胆固醇和甘油三酯与高血压无相关性;高密度脂蛋白(HDL)和体质量指数(BMI)是影响高血压的保护因素,低密度脂蛋白(LDL)水平是影响高血压的危险因素。KCNJ11多态性位点rs2285676在高血压组中基因型TT、CT、CC及等位基因T、C频率分别为50.00%、48.41%、1.59%、74.21%和25.79%。高血压组与对照组差异无统计学意义(P>0.05)。结论 KCNJ11基因rs2285676多态性可能与新疆哈萨克族高血压无关。
Objective To investigate the relationship between the single nucleotide polymorphism（SNP）of KCNJ11（inwardly rectifying potassium channel,subfamily J,member 11）gene and essential hypertension（EH）in Xinjiang Kazak population.Methods The polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） method was used to detect genotypes of rs2285676 polymorphism of KCNJ11,including 126hypertensives（EH group）and 126 normotensives（NT group）in Xinjiang Kazak population.Multiple-factor unconditional Logistic regression analysis was used to evaluate the risk factors for hypertension in Xinjiang Kazak.Results Logistic regression analysis showed that KCNJ11-rs2285676 genotypes,sex,weight,total cholesterol and triglyceride were not associated with hypertension;body mass index（BMI）and high-density lipoprotein（HDL）were protective factors affecting hypertension while low-density lipoprotein（LDL） was a risk factor for hypertension.Genotypes（TT,CT and CC）and allele frequencies（T and C）of the SNP of rs2285676 in KCNJ11 gene were 50.00%,48.41%,1.59%,74.21% and 25.79% in EH group,respectively.The differences between EH and normal control were not significant（P〉0.05）.Conclusion The rs2285676 polymorphism of KCNJ11 is not associated with EH in Xinjiang Kazak population.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2015年第6期792-795,共4页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
国家重点基础研究发展计划项目(973计划)(No.2012CB26600)
兵团博士基金项目(No.2010JC16)
兵团医药专项(No.2012BA021)~~
