摘要
目的探讨新疆维吾尔族银屑病患者表皮中p16基因启动子区甲基化与银屑病发病的关系。方法采用焦磷酸测序技术检测18例维吾尔族银屑病患者皮损组织(银屑病组)与12例正常皮肤组织(对照组)p16基因启动子区CpG岛的甲基化检测率,并比较2组甲基化发生率。结果银屑病组p16基因平均甲基化检测率(16.0%)明显高于对照组(2.75%),差异有统计学意义(P<0.05);银屑病组CpG1、CpG2、CpG3和CpG4甲基化发生率均为100%,对照组分别为100%、25%、0、16%,2组CpG2、CpG3和CpG4甲基化率比较差异均有统计学意义(P<0.05)。结论 p16基因启动子区甲基化状态与新疆维吾尔族银屑病发病有相关性。
Objective To investigate the correlation between the promoter methylation of p16 gene and psoriasis in Xinjiang Uygur patients. Methods The detection rate of methylation of CpG islands in promoter region of p16 gene was detection by pyrosequencing technique in 18 Xinjiang Uygur patients with psoriasis （psoriasis group） and 12 patients with normal skin （control group）. The incidence of methylation was compared between two groups. Results The average detection rate of methylation was significantly higher in psoriasis group （16.0%） than that in control group （2. 75%） （P〈0.05）. The incidences of CpG1, CpG2, CpG3 and CpG4 methylation were 100%, 100%, 100% and 100% in psoriasis group, and were 100%, 25%, 0 and 16% in control group, showing significant differences in CpG2, CpG3 and CpG4 between two groups （P〈0.05）. Conclusion The methylation of p16 gene promoter is correlated with psoriasis in Xinjiang Uygur patients with psoriasis.
出处
《中华实用诊断与治疗杂志》
2015年第11期1082-1083,1087,共3页
Journal of Chinese Practical Diagnosis and Therapy
基金
新疆维吾尔自治区人民医院基金(20120106)
