摘要
为优化超声波协同酶法提取鸡油菌子实体中多糖的工艺条件,在单因素实验的基础上,首先采用PlackettBurman实验设计筛选出影响鸡油菌多糖得率的四个主要因素:酶解p H、固液比、超声功率和酶解时间。随后以鸡油菌多糖得率为响应值,采用Box-Behnken实验设计及响应面分析法优化提取条件,得到最佳提取工艺参数为:酶解p H5.5、固液比1∶46(g/m L)、超声功率210 W、酶解时间68 min。在此条件下多糖理论得率为12.08%、实际得率为11.91%、相对误差1.41%,表明Plackett-Burman设计结合响应面分析法可以很好地对鸡油菌多糖超声协同酶法提取工艺进行优化,可为鸡油菌多糖的广泛应用提供一定的技术支撑。
To optimize the ultrasinic- assisted enzymatic extraction of polysaccharides from Cantharellus cibarius,on the base of the single factor experiment results,enzymatic hydrolysis p H,solid- liquid ratio,ultrasonic power and enzymatic hydrolysis time were identified as main factors that influence polysaccharides yield with the Plackett-Burman experiment design firstly.Then the Box- Behnken experiment design and response surface analysis were adopted to optimize the extraction conditions with polysaccharides yield as the response value. The optimized technological parameters were as follows： p H5.5,solid- liquid ratio 1 ∶ 46（ g / m L）,ultrasonic power 210 W and enzymatic hydrolysis time 68 min. Under these conditions,the theoretical and actual crude polysaccharides yield were 12.08% and 11.91%,respectively,with a relative error of 1.41%,which indicated that the combination of Plackett- Burman design and response surface analysis can well optimize the extraction technology of polysaccharides from Cantharellus cibarius with the ultrasinic- assisted enzymatic extraction method and it can provide certain technical support for the widely used of Cantharellus cibarius.
出处
《食品工业科技》
CAS
CSCD
北大核心
2015年第21期242-248,共7页
Science and Technology of Food Industry
基金
云南省科技计划青年项目(2014FD008)
云南省省级人培项目(KKSY201305002)
