摘要
目的:分析miR-19a、miR-19b在非小细胞肺癌中的表达及其与临床病理特征的关系。方法:非小细胞肺癌手术标本61例及其癌旁正常组织,提取总RNA,采用实时定量PCR方法检测miR-19a、miR-19b在非小细胞肺癌及其癌旁正常组织中的表达,并分析其与临床病理特征的关系。结果:miR-19a、miR-19b在非小细胞肺癌组织中的表达高于对应的癌旁正常肺组织,其表达与临床分期、病理类型及淋巴结有无转移相关(P<0.05)。而在不同年龄、性别和吸烟史患者间差异无统计学意义(P>0.05)。结论:miR-19a、miR-19b高表达与非小细胞肺癌的临床分期、病理分型及有无淋巴结转移密切相关,miR-19a、miR-19b有可能成为非小细胞肺癌的重要肿瘤标志物之一。
Objective：To investigate the expression of miR - 19a and miR - 19b and its association with clinico- pathologic features in non - small cell lung cancer （NSCLC）. Methods： Real time - PCR was used to analyze the ex- pression of miR - 19a and miR - 19b in the samples,and the correlations of miR - 19a and miR - 19b expression to the clinicopathological features were analyzed. Results： The expression of miR - 19a and miR - 19b in non - small cell lung cancer was much higher than in the adjacent normal tissues, and it also showed significant correlation with histology ,tumor node metastasis stage and lymph node metastasis （P 〈 0.05 ） , and the expression of miR- 19a and miR- 19b was not associated with the patients/gender, age, smoking history （P 〉 0.05 ）. Conclusion： The high ex- pression of miR - 19a and miR - 19b expression is closely related to histology, tumor node metastasis stage and lymph node metastasis of NSCLC patients ,miR - 19a and miR - 19b can potentially be used as one of valuable tumor markerin NSCLC.
出处
《现代肿瘤医学》
CAS
2015年第21期3078-3081,共4页
Journal of Modern Oncology
基金
陕西省科技攻关资助项目(编号:2012K13-02)
