摘要
目的探讨上调基因11(URG11)在肝细胞癌组织中的表达及对人肝癌细胞生长、增殖的影响。方法免疫组织化学检测肝细胞癌组织、对应癌旁组织及正常肝组织中URG11的表达水平;反转录PCR和Western blot法检测肝癌细胞系及对照细胞中URG11的表达,分别将URG11基因真核表达载体及URG11 siRNA载体转染人正常肝细胞QZG细胞及肝癌HHCC细胞,MTT法检测转染细胞的增殖情况,流式细胞术检测转染细胞周期变化。结果肝癌组织、癌旁组织及正常肝组织中URG11表达阳性率分别为62.35%、69.41%和18.5%;与正常肝组织相比,肝癌和癌旁组织中URG11水平显著升高。在HHCC肝癌细胞中URG11水平最高,QZG细胞中URG11为阴性。上调QZG细胞中URG11表达水平后,细胞生长加快;下调HHCC细胞中URG11表达后,细胞生长显著减慢。结论 URG11可促进肝癌细胞的生长与增殖,在肝癌的发生中起着重要作用。
Objective To detect the expression of upregulated gene 11 （URG11 ） in hepatocellular carcinoma （HCC） tissues and cell lines and study the effect of URG11 on HCC cell growth. Methods. URG11 expression was detected through immunohistochemistry in normal liver tissues, HCC tissues, and matched adjacent non-cancer tissues. URG11 expression in HCC cell lines and control liver cell line was examined by reverse transcription PCR and western blotting. URG11 eukaryotic expression vector and siRNA vector were transfected into QZG cells and HCC cells. Growth of the transfected cells was investigated by MTT assay. Cell cycle was detected by flow cytometry. Results Positive rates of URG11 expression in HCC tissues was 62.35%, in adjacent non-tumor liver tissues was 69.41%, and in normal liver tissues was 18.5%. URG11 expression was higher significantly in HCC tissues as well as adjacent non-cancer tissues than in normal liver tissues. HCC cells were found to be with the highest URG11 expression, while QZG cells were with negative expression. After URG11 expression was upregulated in QZG cells, QZG cell growth was accelerated, while when URG11 was downregulated in HCC cells, HCC cell growth was suppressed. Conclusion URG11 could promote HCC cell growth and proliferation. It might play an important role in HCC development.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2015年第11期1523-1527,共5页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金(30900675)
