摘要
目的评价缺血后处理对CPB犬肺缺血再灌注损伤时NF-kB及细胞间黏附分子-1（ICAM-1）表达的影响，探讨缺血后处理的肺保护作用及其机制。方法健康成年杂种犬12只，体重12～15kg，雌雄不拘，采用随机数字表法分为CPB组和缺血后处理组（IPO组），每组6只。开胸建立CPB，IPO组于开放左肺动脉即刻行缺血后处理（灌注5min，停灌5min，重复2次）。于CPB前（T1）、开放左肺动脉前（T2）及停机后2h（T3）时取肺组织，光镜下观察病理学结果并行肺损伤评分；采用Westernblot法检测左肺组织NF-kB及ICAM—l蛋白表达；并测定左肺湿／干重比（W／D比）；于T1,3时采集股动脉血样行动脉血气分析，计算氧合指数（OI）、呼吸指数（RI）和肺顺应性（Cdyn）。结果与T1时比较，2组T3时0I和Cdyn降低，RI升高，T2,3时W／D比、肺损伤评分、NF-kB及ICAM-1表达水平升高（P〈0．05）；与T，时比较，T，时2组W／D比、肺损伤评分、NF-kB及ICAM一1表达水平升高（P〈0．05）。与CPB组比较，IPO组T，时OI和Cdyn升高，RI、W／D比、肺损伤评分、NF-kB及ICAM-1表达水平降低（P〈0．05）。结论缺血后处理通过抑制NF-KB活性，下调ICAM-1表达，减轻CPB犬肺缺血再灌注损伤，改善肺功能。
Objective To evaluate the effects of ischemic postconditioning on nuclear factor kappa B （NF-KB） and intercellular adhesion molecule-1 （ICAM-1） expression during lung ischemia-reperfusion （I/R） injury in a dog model of cardiopulmonary bypass （CPB） , and further investigate the pulmonary pro- tection induced by ischemic postconditioning and the underlying mechanism. Methods Twelve adult mon- grel dogs of both sexes, weighing 12-15 kg, were randomly divided into either CPB group or isehemic posteonditioning group （IPO group） using a random number table, with 6 dogs in each group. CPB was es- tablished after the chest was opened in dogs anesthetized with pentobarbital sodium. Ischemic postcondition- ing was induced by 2 cycles of 5 min reperfusion followed by 5 min ischemia immediately after occlusion of the left pulmonary artery was released in group IPO. Before CPB （T1 ） , before occlusion of the artery was released （T2） , and at 2 h after termination of CPB, lung specimens were obtained for examination of path- ological changes which were scored （with light microscope） and for determination of the expression of NF- KB and ICAM-1 （ using Western blot） and wet/dry lung weight ratio （ W/D ratio） in left lung tissues. Blood samples were collected from femoral arteries at T1 and T3 for blood gas analysis, and oxygenation index （OI）, respiration index （RI） and dynamic lung compliance （Cdyn） were calculated. Results OI and Cdyn were significantly decreased, and RI was increased at T3 , and W/D ratio, pathological scores, and expression of NF-KB and ICAM-1 were increased at T2.3 than at T7 in the two groups. W/D ratio, pathologi cal scores, and expression of NF-KB and ICAM-1 were significantly higher at T3 than at T2 in the two groups. Compared with group CPB, OI and Cdyn were significantly increased, and RI, W/D ratio, patho- logical scores, and expression of NF-KB and ICAM-1 were decreased at T3 in group IPO. Conclusion Is- chemic postconditioning up-regulates the exp
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2015年第7期868-871,共4页
Chinese Journal of Anesthesiology
基金
贵州科技厅联合基金资助（黔科合J字LKZ[2012]05）
关键词
心肺转流术
肺
再灌注损伤
NF—kB
胞间黏附分子1
缺血后处理
Cardiopulmonary bypass
Lung
Reperfusion injury
NF-kappa B
Intercellular adhesion molecule-l
Ischemic postconditioning
