摘要
为研究口服槟榔（Areca catechu Linnaeus）仁汁液的毒性,进行了小鼠的经口急性毒性试验。根据预试验确定正式试验给药剂量的范围,选用KM小鼠经口灌胃,一次给药,给药后连续观察7 d,记录小白鼠的中毒反应及死亡情况,采用寇氏法计算LD50及LD50的95%可信限。结果表明,高剂量给药组小白鼠出现中毒反应并死亡,解剖后检查发现主要原因是胃肠和肺部的病变,测得槟榔仁汁液的LD50及LD50的95%可信限分别为12.425 g/（kg.bw）、10.610~14.551 g/（kg.bw）,表明槟榔仁汁液可以被认为是实际无毒物质。槟榔仁原液干物质的LD50及LD50的95%可信限分别为1.349 g/（kg.bw）、1.152~1.580 g/（kg.bw）,属于低毒物质。
The acute oxicity was tested in mice in order to study the oral toxicity of the juice of betel nut seeds. According to preliminary experiments to determine the range of the official test administered dose,KM mice were selected and administered drug by intragastric infusion at single dose,the toxic effect and death condition of the mice were observed for 7days after being administered, and the method of korbor was used to determine LD50 and 95% confidence level. The results showed that the high dose of betel nut juice to mice appeared toxic reactions and death, and the main reason was gastrointestinal and lung disease after autopsy;low dose group was no pathological change in viscera in mice. The LD50 of betel nut juice in mice was 12.425 g / kg and its 95% confidence level was 10.610~14.551 g / kg,which indicated that the betel nut juice was actual non-toxic substances. The LD50 of dry matter of betel nut juice was 1.349 g / kg and its 95% confidence level was 1.152~1.580 g / kg and it was considered the drug of low toxicity.
出处
《湖北农业科学》
2015年第18期4532-4534,4543,共4页
Hubei Agricultural Sciences
基金
海南省中药现代化专项(ZY201417)
海南省三亚市功能槟榔研究重点实验室项目(L1411)
海南省三亚市院地科技合作项目(2014YD16)
海南省三亚市农业科技创新项目(2014NK28)
