摘要
基于生产实践,对非洲菊扩繁生产中的继代增殖培养、壮苗生根培养、瓶栽移栽基质及育苗方式进行研究,结果表明:在增殖培养阶段将KT、BA两种激素交替使用或将KT、BA结合使用可有效避免由于激素使用不当,过早引起种苗弱化甚至玻璃化,可使大批量中间繁殖体在继代过程中稳定增殖;切割操作以"去叶留1/2基部愈伤组织丛芽小块"为非洲菊继代增殖的最佳操作方式;继代转接后前3 d不进行光照可有效节约15%左右的光照耗能;降低细胞分裂素和提高生长素用量,可达到壮苗和增殖同步的效果,也有利于分离切割提高工作效率和之后的生根培养;生根培养中适宜培养基为1/2 MS+IAA 0.5 mg/L+IBA 0.5 mg/L+AC 1.0 g/L;移栽培养宜采用湿润育苗技术。
Based on production practice,three aspects of expanding propagation production of gerbera were studied. Experi-mental results showed that in the multiplication culture stage,interchangeably using two hormones KT and BA or combine u-sing KT and BA could effectiVely aVoid weaken and Vitrification of seedlings at too early stage caused by improper use of hor-mone,and could make large quantities of intermediate propagule stable proliferation during the subculture process. The best practices of subculture multiplication of gerbera was cutting operations to＆quot;remoVe leaVes and remain small pieces of 1/2 bas-al callus clump buds＆quot;,and without lighting can effectiVely saVe about 15% of light energy in the first 3 d after transgenera-tional transfer. Lower cytokinin and increase dosage of auxin could achieVe the effect of the synchronous seedling and prolif-eration and was beneficial to separate cutting to improVe work efficiency and rooting culture,and the suitable medium for roo-ting culture was 1/2MS＋IAA0. 5 mg/L＋IBA0. 5 mg/L＋AC1. 0 g/L,and during transplanting culture,the technology of moisture seedling cultiVation was used.
出处
《福建农业科技》
2015年第8期15-19,共5页
Fujian Agricultural Science and Technology
关键词
非洲菊
组织培养
快繁技术
Gerbera
tissue culture
rapid propagation technique
