摘要
目的分离并鉴定银杏内生真菌产生的抗菌活性物质。方法在一株镰刀菌属内生真菌的发酵液中检测出了较强的抗革兰阳性菌的活性,抗菌活性筛选的检定菌为短小芽孢杆菌[CMCC(B)63202]。发酵液经液液萃取、硅胶柱层析、高效液相分离得到化合物A和B。液相色谱条件:以Shim-pack C_(18)(250mm×10mm,5μm)为固定相,以甲醇-水(5:6)为流动相,流速1.0m L/min,检测波长254nm,进样量20μL。结果经结构解析,化合物A的分子量为639,由3个α-羟基-β-甲基-丁酸(Hiv)与3个N甲基缬氨酸(MeVal)相互间隔地缩合成一个环状对称结构,与文献中恩镰孢菌素B的结构一致。化合物B的分子量为653,与恩镰孢菌素B相差一个CH_2,推测与恩镰孢菌素B_1或恩镰孢菌素D同质。结论该银杏内生真菌产生的抗菌活性物质为恩镰孢菌素。
Objective To isolate and identify antimicrobial substances from endophytic fungus of Ginkgo biloba L. Methods The substances against gram-positive bacteria were discovered in fermentation broth from a strain endophytic fungus of Fusarium spp. The test strain of antibiotics was Bacillus pumilus [CMCC（B）63202]. Compound A and B were obtained, by the separation processes, including liquid-liquid extraction, silica-gel column chromatography and high performance liquid chromatography（HPLC）. The HPLC system was performed on a Shim- pack C18 column （250mm× 10mm, 5μm）, with methanol- water （5：6） as mobile phase, and flow rate was 1.0 mL/min, UV detector at 254nm. Results By several structure analysis methods, compound A was consistent with enniatin B in report that a symmetrical cyclic structure was condensed by 3 pieces of α-hydroxyl-β-methyl-butyric acid （Hiv） and 3 pieces of N-methyl-valine （Me-Val） in turn, and its molecular weight was 639. Compound B was estimated to enniatin B1 or enniatin D, because its molecular weight was 653 and different of CH2 with enniatin B. Conclusion The antimicrobial substances from the strain endoohvtic fungus were enniatins.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2015年第10期728-731,共4页
Chinese Journal of Antibiotics
关键词
银杏
内生真菌
镰刀菌属
恩镰孢菌素
Ginkgo biloba L.
Endophytic fulagus
Fusarium spp.
Enniatins
