摘要
目的探讨山楂叶总黄酮对慢性脑缺血大鼠脑组织p38蛋白表达的影响。方法健康雄性SD大鼠,随机分为假手术组、模型组、山楂叶总黄酮组、银杏叶片组。采用永久性双侧颈总动脉结扎法制备慢性脑缺血模型。Morris水迷宫检测大鼠学习记忆能力;Western blot法检测p38蛋白的表达。结果与模型组比较,山楂叶总黄酮可明显改善血管性痴呆大鼠学习记忆能力,缩短大鼠逃避潜伏期(P<0.05),增加大鼠穿越平台次数(P<0.05),抑制大鼠缺血脑组织p38蛋白的表达(P<0.05)。结论山楂叶总黄酮对慢性脑缺血大鼠的神经功能具有保护作用,其机制可能与抑制p38蛋白表达,减轻脑神经元凋亡有关。
Objective To investigate the therapeutic effect and mechanism that total flavone of Hawthorn leaf( TFHL)on p38 protein in brain of the chronic cerebral ischemia rat. Methods Male healthy SD rats were randomly divided into sham group,model group,TFHL group and Ginkgo leaf group. Permanent bilateral carotid artery ligation was used to prepare chronic cerebral ischemia model. Morris water maze method was used to evaluate learning and memory abilities of rats;Western blot method was used to measure the expression of p38 protein( P < 0. 05). Results Compared with the model group,TFHL treatment can improve learning and memory capabilities of vascular dementia rats,shorten the escape latency( P < 0. 05),increase the number of crossing the platform( P < 0. 05) and dramatically inhibit the expression of p38 protein( P < 0. 05). Conclusion TFHL played a protective role to nerve function of the chronic cerebral ischemia rat. The mechanism of its anti- apoptosis might be associated with down- regulating expression of p38 protein,reduced the apoptosis of neuron in the brain.
出处
《药学研究》
CAS
2015年第11期628-630,647,共4页
Journal of Pharmaceutical Research
基金
河北省科技厅指令课题(No.15277789D)
河北省教育厅青年基金指令课题(No.QN2014103)
河北省高校省级重点学科资助项目(No.2013)
河北省中医药抗痴呆重点研究室建设项目资助(No.2014-2017)
承德医学院大学生科研重点资助项目(No.2015005)
