摘要
从大连海域采集的海泥样品中筛选能产碱性蛋白酶的海洋细菌。用酪蛋白平板涂布法筛选得到55株具有产碱性蛋白酶能力的菌株。分离纯化后,经酪蛋白平板点种法初筛和福林酚法复筛,得到3株具有较强产碱性蛋白酶能力的菌株DL07、DL18和DL31,其酶活分别为156.5 U/m L、129.7U/m L和179.4 U/m L。实验结果表明从大连海域获得了碱性蛋白酶高产菌株,丰富了菌种资源。菌株DL07和DL31将来可作为良好的出发菌株进行改良、驯化,探索产物碱性蛋白酶优良特性,以期适用于工业应用。
To Isolate the alkaline protease-producing marine bacteria from sea mud samples of Dalian sea area, 55 bacteria which can product alkaline protease were obtained from Casein media using spread-plate method. And after the separation and purification, 3 strains called DL07, DL18 and DL31 with stronger enzyme production capacity have been got by size comparison of the hydrolysis zone and by rescreening by Folin-phenol reagent method. And their enzyme activity were 156.5 U/m L, 129.7U/m L and 179. 4U/m L. It shows that we can get alkaline protease-producing marine bacteria from Dalian sea area, and the species resources may be enriched. Strain DL07 and DL31 may be improved and domesticated as the good starting strains.
出处
《生物技术世界》
2015年第10期36-37,共2页
Biotech World
基金
大连民族大学博士启动金
中央高校基本科研业务费专项资金(DC201502020401)
大学生创新创业训练计划(X201403060)
关键词
碱性蛋白酶
海洋菌
筛选
alkaline protease
marine bacteria
isolation
