摘要
[目的]研究菌株I13产碱性蛋白酶的酶学性质。[方法]从一株碱性蛋白酶生产菌株I13中得到了碱性蛋白酶粗酶液,并研究了温度和pH对该酶液酶活力的影响。[结果]所产碱性蛋白酶的最适反应温度为40℃,在20~30℃范围内仍保持了最大活力的40%以上;最适反应pH为10.5,在pH 8.0~11.0范围内保持了最大活性的90%以上,有较宽的pH谱。[结论]该碱性蛋白酶具有开发成为洗衣粉添加剂的巨大潜力。
[Objective] This study was aimed to investigating properties of alkaline protease produced by strain I13.[Method] Crude enzyme of alkaline protease was obtained from alkaline protease produced by strain I13,while effects of temperature and pH value on enzyme activity was also investigated in this study.[Result] The optimal temperature of alkaline protease produced by strain I13 was 40 ℃,and over 40% of the largest enzyme activity still maintained within the range from 20 to 30 ℃.The optimal pH value was 10.5,and over 90% of the largest enzyme activity still maintained within the range from 8.0 to 11.0,which had broader pH value spectrum.[Conclusion] This alkaline protease has huge potential to be developed into washing-powder additive.
出处
《安徽农业科学》
CAS
北大核心
2011年第13期7574-7575,共2页
Journal of Anhui Agricultural Sciences
基金
国家自然科学基金项目(30670067)
关键词
碱性蛋白酶
筛菌
酶学性质
Alkaline protease
Screening
Enzymatic characterization
