摘要
目的探讨Wnt信号通路激活在肝门部胆管癌发生中的作用。方法回顾性分析1998年7月至2007年5月在中山大学附属第一医院行手术切除治疗的129例肝门部胆管癌及45例先天性胆总管囊肿患者临床资料。其中肝门部胆管癌患者男91例,女38例;平均年龄(56±23)岁。先天性胆总管囊肿患者男32例,女13例;年龄(39±11)岁。所有患者均签署知情同意书,符合医学伦理学规定。制作患者病理标本的组织芯片,对Wnt信号蛋白(Wnt)-2、β-链蛋白(β-catenin)、T细胞因子-4(TCF-4)蛋白进行免疫组化染色。以Wnt-2、β-catenin和TCF-4 3个指标同时阳性表达为Wnt信号通路激活。两组率的比较采用χ2检验。结果免疫组织染色结果显示,肝门部胆管癌组织Wnt-2阳性表现为细胞浆出现棕黄色颗粒,β-catenin、TCF-4阳性表现为细胞核出现棕黄色颗粒。肝门部胆管癌组织中Wnt-2、β-catenin和TCF-4 3个指标同时阳性表达的发生率为54%(70/129),明显高于先天性胆管囊肿的29%(13/45)(χ2=11.2,P<0.05)。结论 Wnt信号通路激活可能是肝门部胆管癌发生的重要分子生物学机制之一。
Objective To investigate the effect of Wnt signaling pathway activated on occurrence of hilar cholangiocarcinoma.Methods Clinical data of 129 patients with hilar cholangiocarcinoma and 45 patients with congenital choledochal cyst undergoing surgical resection in the First Affiliated Hospital of Sun Yat-sen University between July 1998 and May 2007 were retrospectively studied.Among the 129 patients with hilar cholangiocarcinoma,91 were males and 38 were females with the average age of(56±23) years old.Among the 45 patients with congenital choledochal cyst,32 were males and 13 were females with the average age of(39±11) years old.The informed consents of all patients were obtained and the local ethical committee approval had been received.The tissue microarray of the pathological specimen was made.Wnt signal protein(Wnt)-2,β-catenin and T cell factor-4(TCF-4) were treated by immunohistochemical staining.The positive expression of 3 indexes Wnt-2,β-catenin and TCF-4 observed simultaneously was considered as activated Wnt signaling pathway.The rate comparison of two groups was conducted using chi-square test.Results According to results of immunohistochemical staining,the positive expression of Wnt-2 in hilar cholangiocarcinoma tissues was observed in the appearance of brown granules in cytoplasm,while the positive expression of β-catenin and TCF-4 was observed in the appearance of brown granules in cell nucleus.The incidence of simultaneous positive expression of the 3 indexes Wnt-2,β-catenin and TCF-4 in hilar cholangiocarcinoma tissues was 54%(70/129),which was significantly higher than 29%(13/45) in congenital choledochal cyst(χ2=11.2,P<0.05).Conclusion The activation of Wnt signaling pathway may be one of the important molecular biological mechanisms of the occurrence of hilar cholangiocarcinoma.
出处
《中华肝脏外科手术学电子杂志》
CAS
2015年第5期323-326,共4页
Chinese Journal of Hepatic Surgery(Electronic Edition)
基金
广东省自然科学基金(S2013010015386
S2012040006299
10451008901005954)
关键词
胆管肿瘤
信号传导
芯片分析技术
Wnt2蛋白质
Bile duct neoplasms
Signal transduction
Microchip analytical procedures
Wnt2 protein
