摘要
目的:探讨半乳糖凝集素-3(galectin-3,Gal-3)在宫颈癌组织中的表达情况及其临床意义。方法:采用组织芯片和免疫组织化学法,半定量检测174例接受根治性手术治疗的Ⅰ～Ⅱ期子宫颈癌患者肿瘤组织中Gal-3蛋白的表达情况,并分析其与患者临床病理特征、治疗效果及预后的关系。结果:宫颈癌组织中Gal-3的表达阳性率为54.0%(94/174)。Gal-3蛋白阳性表达与非鳞癌(P=0.006)、淋巴血管间隙侵犯(P=0.048)、深肌层浸润(P=0.001)以及盆腔淋巴结转移(P<0.001)均显著相关,并且是预测盆腔淋巴结转移的独立危险因素之一。而FIGO分期晚(P<0.001)、淋巴血管间隙侵犯(P=0.013)、宫旁浸润(P=0.015)、盆腔淋巴结转移(P<0.001)和Gal-3阳性表达(P=0.013)的患者总生存率较低。术前放疗后,Gal-3蛋白表达增加的患者局部肿瘤缓解率(39.7%)显著低于Gal-3表达降低或不变的患者(70.0%)。结论:Gal-3蛋白的阳性表达与宫颈癌患者盆腔淋巴结转移及预后差有关。Gal-3蛋白的表达增加可能导致肿瘤对放疗产生耐受。
Objective：To evaluate the expression of galectin-3（Gal-3） in cervical cancer tissues and its correlation with clinicopathological and survival variables.Methods：One hundred and seventy-four patients with International Federation of Gynaecology and Obstetrics（FIGO） stage Ⅰ/Ⅱ cervical carcinoma undergoing radical surgery were selected,and their cervical cancer tissues were collected.The expression of Gal-3 protein in cancer tissues was semi-quantitatively tested by tissue microarray and immunohistochemical analysis.Then the correlations of the expression of Gal-3 with the clinicopathological parameters,treatment outcomes and the survival of these patients were evaluated.Results：The positive expression rate of Gal-3 was 54.0%（94/174）in the cervical cancer tissues.In a univariate analysis,the positive expression of Gal-3 was closely correlated with non-squamous cell carcinoma（P=0.006）,lymph vascular-space invasion（LVSI,P=0.048）,deep-muscle invasion（P= 0.001） and pelvic lymph-node metastasis（P0.001）.The multivariate analysis revealed that the expression of Gal-3 was one of the independent risk factors for predicting pelvic lymph-node metastasis.The risk factors including FIGO stage Ⅱ（P0.001）,LVSI（P=0.013）,positive parametrium invasion（P=0.015）,pelvic lymph-node metastasis（P0.001） and the positive expression of Gal-3（P=0.013） were significantly correlated with poor overall survival in these patients with cervical cancer.The tumor remission rate was lower in patients with up-regulated expression of Gal-3（39.7%） than that in patients with down-regulated or invariant expression of Gal-3（70.0%） after receiving preoperative radiotherapy.Conclusion：Positive expression of Gal-3 protein is a predictive factor for pelvic lymph node metastasis and worse overall survival in patients with cervical cancer.Up-regulated expression of Gal-3 protein may be associated with the resistance to radiotherapy.
出处
《肿瘤》
CAS
CSCD
北大核心
2011年第2期148-153,共6页
Tumor
基金
国家自然科学基金资助项目(编号:30672417)
关键词
宫颈肿瘤
半乳糖凝集素3
芯片分析技术
肿瘤转移
预后
Uterine cervical neoplasms
Galectin 3
Microchip analytical procedures
Neoplasm metastasis
Prognosis
