摘要
目的对十堰地区2014年分离的35株多重耐药嗜麦芽窄食单胞菌(SMA)进行耐药表型、耐药基因研究。方法收集十堰地区2014年分离的35株多重耐药SMA,采用纸片扩散法检测细菌耐药性。超广谱β-内酰胺酶(ESBLs)采用标准纸片法检测,头孢菌素酶(AmpC)和金属β-内酰胺酶(MBLs)检测采用三维试验,非金属碳青霉烯酶(KPC)检测采用改良Hodge试验。采用PCR法检测细菌耐药基因,对阳性结果进行基因正、反向测序分析。结果 35株多重耐药SMA中,ESBLs、AmpC、MBLs、KPC阳性率分别为45.7%、14.3%、94.3%、0.0%。耐药基因blaTEM、blaSHV、blaCARB、blaIMP、blaOXA-10、oprD2的阳性率分别为22.8%(8/35)、11.4%(4/35)、17.1%(6/35)、94.3%(33/35)、8.6%(3/35)、28.5%(10/35)。结论该地区的多重耐药SMA对碳青霉烯类抗菌药物的耐药状况非常严重,应引起重视。
Objective To study the drug‐resistance phenotype and drug resistance gene in 35 strains of multi‐drug resistant Stenotrophomonas maltophilia（SMA ） in Shiyan area during 2014 .Methods 35 strains of multi‐drug resistant SMA isolated from Shiyan area during 2014 were collected and their drug resistance was detected by using the K‐B method .ESBLs was detected by using the standard paper strip method .AmpC and MBLs were detected by the three‐dimensional test ,KPC was detected by the modified Hodge test .The PCR method was adopted to detect the bacterial drug resistance gene and the positive results were performed the forward and reverse gene sequencing a‐nalysis .Results Among 35 strains of multi‐drug resistance SMA ,the positive rates of ESBLs ,AmpC ,MBLs and KPC were 45 .7% ,14 .3% ,94 .3% and 0 .0% respectively .The positive rates of drug resistance gene blaTEM ,blaSHV , blaCARB ,blaIMP ,blaOXA‐10 and op rD2 were 22 .8% （8/35） ,11 .4% （4/35） ,17 .1% （6/35） ,94 .3% （33/35） ,8 .6% （3/35） and 28 .5% （10/35） respectively .Conclusion The resistance of multi‐drug resistance SMA in this area to carbapenem antibacterial drugs is very serious ,which should arouse the attention .
出处
《检验医学与临床》
CAS
2015年第19期2839-2841,2844,共4页
Laboratory Medicine and Clinic
基金
湖北省卫生计生科研基金资助项目(WJ2015Z115)
湖北省十堰市科技局科技计划立项(15K71)
关键词
医院感染
多重耐药嗜麦芽窄食单胞菌
Β-内酰胺酶
耐药基因
nosocomial infection
drug-resistant stenotrophomonas maltophilia
β-lactamase
drug re-sistant gene
