摘要
目的探讨增殖抑制基因(HSG)与表皮生长因子受体(EGFR)在乳腺癌组织中的表达及与其临床病理因素的相关性。方法应用荧光定量PCR及免疫组织化学方法,检测在正常乳腺组织、乳腺纤维腺瘤组织及乳腺癌组织中HSG及EGFR的mRNA表达,研究两者的相关性。结果乳腺癌组HSG mRNA表达水平显著低于正常乳腺组和纤维腺瘤组(P<0.05),而EGFR mRNA表达水平则显著高于正常乳腺组及纤维腺瘤组(P<0.05)。乳腺癌组织中HSG、EGFR的mRNA表达水平与淋巴结转移、病理组织学分级、雌激素受体和HER-2显著相关(P<0.05)。HSG与EGFR的mRNA表达呈负相关,γ=-0.427,P<0.05。结论乳腺癌组织中HSG、EGFR的mRNA表达与肿瘤组织分化程度、淋巴结转移有关,为患者疾病程度的评估、预后评估及个体化治疗方案的设计提供临床依据。
Objective To study the expression of hyperplasia suppressor gene( HSG) and epidermal growth factor receptor( EGFR),and their correlation with clinicopathological features in breast cancer tissues. Methods Real-time fluorescene quatitative polymerase chain reaction( RT-PCR) and SP immunohistochemistry were applied to detect the mRNA expression of HSG and EGFR in normal breast tissue,breast carcinoma and breast fibroadenoma tissues,and their correlation. Results The mRNA expression of HSG of breast cancer tissues was significantly lower than those of breast fibroadenoma and normal breast tissues( P < 0. 05); The mRNA expression of EGFR of breast cancer tissues was significantly higher than those of breast fibroadenoma and normal breast tissues( P < 0. 05). The mRNA expression of HSG and EGFR was related with lymph node metastasis,different pathological grades of breast cancer,ER and HER-2( P < 0. 05). The mRNA expression of HSG and EGFR were negatively correlated in breast cancer tissues( γ =- 0. 427,P < 0. 05). Conclusion The expression of HSG and EGFR mRNA in breast cancer tissue is associated with the degree of differentiation,lymph node metastasis,and can provide clinical basis for evaluation of the degree of disease,treatment,prognosis and the design of individualized treatment plan.
出处
《实用癌症杂志》
2014年第6期629-632,共4页
The Practical Journal of Cancer
关键词
乳腺癌
增殖抑制基因
表皮生长因子受体
Breast cancer
Hyperplasia suppressor gene
Epidermal growth factor receptor
