摘要
目的：研究酪氨酸216位点磷酸化糖原合成酶激酶－3β〔p －GSK －3β（Try216）〕在肥胖多囊卵巢综合征（PCOS）患者卵巢颗粒细胞中的表达。方法选择因 PCOS、不孕行体外受精／单精子卵胞浆内注射－胚胎移植（IVF／ICSI －ET）取卵的患者10例，其中 PCOS 肥胖组〔体质指数（BMI）≥28．0 kg／m2〕5例，PCOS 非肥胖组（18．5 kg／m^2＜BMI≤23．9 kg／m^2）5例；另外选择因输卵管梗阻或男方因素行 IVF／ICSI －ET 取卵者5例作为非PCOS 对照组（18．5 kg／m^2＜BMI≤23．9 kg／m^2）。取卵后收集颗粒细胞进行培养，葡萄糖氧化酶（GOD）法检测第48 h 细胞培养液葡糖糖含量，免疫印迹法检测 p －GSK －3β（Try216）表达。结果非 PCOS 对照组、PCOS 非肥胖组、PCOS 肥胖组中葡萄糖含量依次增高（P ＜0．05），p －GSK －3β（Try216）表达量依次增加（P ＜0．05），差异均有统计学意义。结论PCOS 患者卵巢颗粒细胞中糖代谢异常，肥胖加重 PCOS 患者糖代谢紊乱。 p －GSK －3β（Try216）在肥胖 PCOS 患者糖代谢紊乱中发挥重要作用。
Objective To investigate the level of p -GSK -3β（Try216） in the ovarian granulosa cells of obese pa-tients with polycystic ovarian syndrome （PCOS）.Methods A total of ten patients were enrolled into the current study who underwent in vitro fertilization/intracytoplasmic sperm injection and embyro transfer （IVF/ICSI -ET） due to infertil-ity caused by PCOS.These patients were divided into two groups according to their conditions： an obese PCOS group （BMI≥28 kg/m^2 , n =5） and a non -obese PCOS group （18.5 kg/m^2 〈BMI≤23.9 kg/m^2 , n =5）.Meanwhile, an-other five patients were adopted as a non -PCOS control group （18.5 kg/m2 〈BMI≤23.9 kg/m^2 ） who suffered from blocked fallopian tubes or underwent IVF/ICSI -ET due to male infertility.Granulosa cells were collected from patients during IVF/ICSI -ET.The production of glucose in the culture medium was measured by GOD method after 48 h of culti-vation.The quantity of p -GSK -3β（Try216） in the ovarian granulosa cells was detected by Western blotting analysis. Results The levels of glucose and p -GSK -3β（Try216） was highest in the obese PCOS group, followed by the non -obese PCOS group and then the non -PCOS group （P 〈0.05）.Conclusion Abnormal glucose metabolism is present in the ovarian granulosa cells of PCOS patients, which can be aggravated by obesity.The protein p -GSK -3β（Try216） is essential for the glucose metabolism of obese patients with PCOS.
出处
《徐州医学院学报》
CAS
2015年第8期524-527,共4页
Acta Academiae Medicinae Xuzhou
