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氯胺酮对老年大鼠腹壁疝术后炎症的影响及作用机制的探讨

The effect of ketamine on ventral hernia repair in elderly rat
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摘要 目的观察小剂量氯胺酮对老年腹壁疝大鼠术后炎性细胞因子释放的影响及作用机制的探讨。方法选用老年雄性SD大鼠,腹正中切口去除腹壁的肌层、筋膜和腹膜后直接缝合皮肤,一周后将腹壁疝SD大鼠60只随机分为四组:分别为对照组、手术组、KTM(氯胺酮)组和手术+KTM组,其中手术组采用补片进行腹壁疝修补术(IPOM)。术后处死,断头取脑组织。四组中白介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)的表达量用RT-PCR来检测;总糖原合成酶激酶-3β(GSK-3β)和磷酸化GSK-3β(p-GSK-3β)的蛋白表达水平用Western blotting方法分别检测。结果与对照组相比,手术组和手术+KTM组的IL-1β、TNF-α和p-GSK-3β的表达量明显增加(P<0.05);KTM组中上述各项指标无明显变化(P>0.05)。手术+KTM组中的炎性因子(IL-1β和TNF-α)表达量与手术组相比显著降低,而p-GSK-3β表达量则比手术组显著增高(P<0.05),GSK-3β总的表达量在各组之间无统计学差异。结论小剂量的氯胺酮可以减轻老年大鼠腹壁疝修补术后脑组织中的炎性因子(IL-1β和TNF-α)的表达水平,且此作用可能与GSK-3β的活性有关,对预防老年术后认知功能障碍具有一定的指导意义。 Objective To investigate the effect and mechanism of ketamine on ventral hernia repair in aged rat. Methods Skin is sutured after removing the abdominal wall muscle,fascia and peritoneum skin on abdominal incision for aged male SD rats. A week later,the abdominal wall hernia 60 SD rats were randomly divided into four groups: control group,surgery group,KTM group and surgery + KTM group.Ventral hernia repair operation was performed in surgery group( IPOM). Brain tissue were collected after decapitation. Interleukin( IL)-1β and tumour necrosis factor( TNF)-α were examined by Reversetranscription polymerase chain reaction( RT-PCR). Protein expression of total glycogen synthase kinase-3β( GSK-3β) and phosphorylation of GSK-3β( p-GSK-3β) were analyzed by western blotting. Results Compared with control group,the production of IL-1β,TNF-α and p-GSK-3β significantly increased in surgery group and surgery + ketamine group( P〈0. 05). Otherwise,ketamine group had no influence on the above expression. Compared with surgery group,the production of IL-1β and TNF-α significantly decreased in surgery + ketamine group, and phosphorylation of GSK-3β significantly increased. Conclusion Ketamine inhibits ventral hernia repair inflammation( IL-1β and TNF-α) in aged rats possibly via GSK-3βpathway. And this effect may be has certain significance guiding in the prevention of POCD for elderly patients.
作者 宿明艳 张凯
出处 《中华疝和腹壁外科杂志(电子版)》 2016年第2期134-136,共3页 Chinese Journal of Hernia and Abdominal Wall Surgery(Electronic Edition)
关键词 腹壁疝 氯胺酮 炎性因子 糖原合成酶激酶-3Β Ketamine Ventral hernia Inflammatory cytokines Glycogen synthase kinase-3β
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