摘要
目的探讨阿魏酸(Ferulic acid,FA)对不同时间点糖氧剥夺(oxygen-glucose deprivation,OGD)诱导脑微血管内皮细胞(brain microvascular endothelial cells,BMECs)氧化损伤的保护作用。方法采用混合气体的方法(95%N2+5%CO2)建立OGD体外模型,通过MTT法检测细胞活力、流式细胞术检测活性氧(reactive oxygen species,ROS)产生量,硫代巴比妥酸显色法检测细胞丙二醛(methane dicarboxylic aldehyde,MDA)水平,氮蓝四唑显色法检测细胞总超氧化物歧化酶(superoxide dismutase,SOD)活性。结果 OGD对BMEC细胞造成的氧化损伤程度呈时间依赖性,FA能显著提高被损伤的细胞存活率,减少ROS的生成量,明显降低细胞内MDA的水平,并提高SOD活性。结论 FA对不同时间点OGD诱导BMEC细胞氧化损伤具有良好的保护作用,其作用机制可能与缓解细胞氧化应激状态有关。
Objective To investigate the protective effects of ferulic acid （FA） on oxidative injury of brain microvascular endothelial cells （BMECs） induced by oxygen-glucose deprivation （OGD） at different time points. Methods The invitro model of OGD was established with mixed gas（95 % N2 ＋ 5 % CO2）. Cell viability was determined by MTT assay. Reactive oxygen species （ROS） generation was assessed by flow cytometry. Cellular malondialdehyde （MDA） was evaluated by glucosinolates barbituric acid chromogenic kit. Superoxide dismutase （SOD） activity was measured by nitroblue tetrazolium chromogenic kit. Results BMECs were damaged by OGD in a time-dependent manner. Compared to the OGD group, FA significantly increased the viability of BMECs, reduced ROS generation and MDA level, and improved the SOD activity （P 〈 0.01 ）. Conclusion FA has considerable protective effects on OGD-impaired BMECs at different time points. The potential mechanism might be related to the amelioration of OGD-induced oxidative stress and damage
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2015年第5期622-626,共5页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
国家自然科学基金(No.81273817
No.81473740)
广东省自然科学基金(No.2014A030313399)
广州市科技计划项目(No.201510010003)
华南中医药协同创新中心-中医药防治脾胃病
脑病创新研究团队项目(No.A1-AFD01514A05)
关键词
阿魏酸
缺糖缺氧
氧化损伤
内皮细胞
Ferulic acid
Oxygen-glucose deprivation
Oxidative injury
Endothelial cells
