摘要
目的报道一例由口普雷沃菌引起的女性患者生殖道脓肿术后血流感染病例。方法无菌方法采集血样本,使用Versa TREK240-10全自动微生物培养检测系统进行培养。血培养报阳后进行涂片镜检、转种培养,分离致病菌,采用法国生物梅里埃公司VITEK 2 Compact全自动细菌分析仪进行鉴定,同时用16S rRNA测序鉴定致病菌。结果培养至48和72 h,血培养仪分别报告左侧、右侧厌氧血培养阳性。VITEK 2 Compact全自动细菌分析仪和16S rRNA测序鉴定结果均为口普雷沃菌,鉴定率分别为96%和99%。结论从上海市东方医院收治的一例女性患者术后血样本中分离出一株口普雷沃菌。
Objective To report a bloodstream infection case of a female patient after genital tract abscess operation by Prevotella oralis.Methods Blood samples were collected by aseptic method, and were detected by VersaTREK240-10 automatic microbial detection system.The positive culture blood samples were smeared, inoculated of plates for pure isolates of pathogenic bacteria, identified by VITEK 2 Compact, and confirmed by 16S rRNA sequencing.Results Two bottles of left side and right side of anaerobic blood culture were positive after 48 and 72 h, respectively.The results of VITEK 2 Compact and 16S rRNA sequencing results were both Prevotella oralis, and the identification rates were 96%and 99%, respectively.Conclusions A isolate of Prevotella oralis is identified from a female patient with bloodstream infection in East Hospital.
出处
《检验医学》
CAS
2015年第9期958-961,共4页
Laboratory Medicine
基金
上海市浦东新区卫生系统重点专科建设项目(PWZz2013-03)
上海市浦东新区卫生系统学科带头人计划(PWRd2012-09)
关键词
普雷沃菌
普雷沃菌属
血流感染
Prevotella oralis
Prevotella
Bloodstream infection
