摘要
为了探讨滩羊卵母细胞体外成熟过程中促卵泡素(FSH)和促黄体素(LH)的最佳添加量,试验设为4组:对照组、FSH组(10、50、100、200、300μg/m L 5个质量浓度梯度)、LH组(5、10、20、50、100μg/m L 5个梯度)和FSH&LH组(100μg/m L FSH+20μg/m L LH),对滩羊卵母细胞的体外成熟培养进行研究,统计第一极体排出率.结果表明:100μg/m L FSH+20μg/m L LH组卵母细胞成熟率最高,为64.64%,明显高于其他3组,差异显著(P<0.05);FSH组中100μg/m L优于其他4个质量浓度梯度和对照组,差异显著(P<0.05);LH组的20μg/m L的质量浓度优于其他4组和对照组,差异显著(P<0.05).因此,在本试验条件下,100μg/m L FSH+20μg/m L LH是滩羊卵母细胞体外成熟培养的最佳培养添加质量浓度.
To investigate the appropriate concentration of FSH and LH in TAN sheep oocyte maturation in vitro, TAN sheep oocytes were divided into four groups in vitro maturation culture： the control group, the FSH group, the LH group and the FSH＆LH group. In the FSH group, the concentration of FSH was 10μg/ml, 50μg/ml, 100μg/ml, 200μg/ml and 300 μg/ml respectively, in the LH group, the concentration of LH was 5μg/ml, 10μg/ml, 20μg/ml, 50μg/ml and 100 μg/ml respectively, and the concentration of FSH and LH were respectively added 100 μg/ml and 20μg/ml in the FSH＆LH group. The rates of polar body I （Pb I） expulsion was analyzed in the test. The results showed that the rate of oocytes maturation of the FSH＆LH group was 64.64%, which was the highest rate in each group （P〈0.05）, the rate of oocytes maturation of the FSH＆LH group was 64.64%, which the highest rate in each group （P〈0.05）, the FSH group including 100 μg/ml is better than the other four concentration gradient and the control group （P〈0.05）, the LH group including 20μg/ml was better than the other four concentration gradient and the control group （P〈0.05）. Therefore, adding 100 μg/ml FSH and 20 μg/ml LH was the appropriate concentration of FSH and LH in TAN sheep oocyte maturation in vitro under this experimental condition.
出处
《农业科学研究》
2015年第3期19-21,28,共4页
Journal of Agricultural Sciences
基金
宁夏自然科学研究基金资助(NZ12150)
项目名称:激素对滩羊卵母细胞体外培养的研究
