摘要
目的探讨重楼皂苷Ⅶ联合顺铂抑制荷瘤小鼠移植瘤生长的分子机制。方法SKOV3细胞皮下接种NOD/SCID小鼠,待移植瘤形成后,荷瘤小鼠随机分为4组(n=10):对照组、低剂量重楼皂苷Ⅶ组(LD组)、中剂量重楼皂苷Ⅶ组(MD组)、高剂量重楼皂苷Ⅶ组(HD组)。15天后,分离肿瘤组织,测定肿瘤体积和重量及抑瘤率;TUNEL染色和Western blot检测肿瘤细胞凋亡及其机制。结果在HD组中,肿瘤重量抑制率达57.98%,体积抑制率达55.11%,细胞凋亡率达42.39%,与对照组相比有统计学差异。经重楼皂苷作用后,凋亡相关基因Bcl-2表达减少,Bax蛋白表达增加;内质网相关基因GRP78和CHOP表达均增加。结论重楼皂苷能有效促进肿瘤细胞凋亡,并且这种凋亡是由内质网应激介导的。
Objective To investigate the molecular mechanism of inhibiting of tumor growth in tumor-bearing mice by polyphyllin Ⅶ and cisplatin.Methods SKOV3 cells were inoculated subcutaneously into NOD/SCID mice,and the tumor-bearing mice were randomly divided into four groups （n = 10）：the control group,low-dose of polyphyllin Ⅶgroup （group LD）,medium-dose of polyphyllin Ⅶ group （group MD）,high-dose of polyphyllin Ⅶ group （group HD）. 15 days later,the tumor tissues were isolated and measured volume and weight to calculate the tumor inhibition rate;the tumor cell apoptosis and its mechanism were determined by TUNEL staining and Western blot.Results In group HD, the weight inhibition rate was 57.98%,volume inhibition rate was 55.11%,and the apoptotic rate was 42.39%,there was a significant difference compared with the control group.After treatment of polyphyllin Ⅶ,the expression of Bcl-2 was decreased and the expression of Bax was increased;the expression of GRP78 and CHOP,the endoplasmic reticu-lum-related gene,was increased too.Conclusion The polyphyllins could effectively promote the apoptosis of tumor cells via endoplasmic reticulum stress.
出处
《中国实验诊断学》
2015年第9期1450-1453,共4页
Chinese Journal of Laboratory Diagnosis
基金
中国博士后基金资助(2013M541316)
吉林省卫生厅科研课题(2013Z097)
关键词
重楼皂苷Ⅶ
移植瘤
凋亡
内质网应激
Polyphyllin Ⅶ
xenografted tumor
apoptosis
endoplasmic reticulum stress
