摘要
从产自四川彭州的华重楼(Parispolyphyllavar·chinensisFranch)地下块茎中分离得到16株菌。经以薯蓣皂甙元为标准对照的薄层层析分析,表明其中编号为SNUS-1(AY842149)的菌株能分泌重楼皂甙或其类似化合物。16SrDNA序列(约1,500bp)系统发育分析表明:菌株SNUS-1与Pseudomonastolassii(AF255336)和Pseudomonastolassii(D84028)的遗传距离分别为0和0·003,在系统进化树上三者又严格聚为一族,结合形态及生理特征确定其为托氏假单孢菌(Pseudomonastolassii)。
Sixteen endophytes were isolated from the hypogenous stems of Paris polyphylla vat. chinensis in Sichuan. Through thin layer chromatography （TLC） with standard comparison of Paris chinensis Franch saponins, the fermentation cultures of strains marked SNUS-1 were identified to contain Paris chinensis Franch saponins or its analogue. A phylogenetic anlysis based on 16S rDNA gene sequences （about 1500bp） shows that the p-distance of the strain SNUS-1 from Pseudomonas tolassii （AF255336） and Pseudomonas tolassii （D84028） are 0 and 0. 003, respectively. These three strains gather into a cluster strictly in the phylogeny tree. Strain SNUS-1 was identified an P. tolassii based on physiological, biochemical and molecular characteristics.
出处
《微生物学通报》
CAS
CSCD
北大核心
2006年第5期84-88,共5页
Microbiology China
关键词
重楼
内生菌
甾体皂甙
16S
RDNA
假单孢菌属
Paris polyphylla var. chinensis , Endophyte, Steroidal saponins, 16S rDNA, Pseudomonas
