摘要
目的研究Survivin拮抗肽对裸鼠脑胶质瘤的抑制效应。方法将体外培养的人胶质瘤U251细胞经立体定向技术注入裸鼠脑尾状核区域,构建荷U251胶质瘤裸鼠原位模型。荷瘤11d后,随机分成实验组和对照组,分别经腹腔注射Survivin拮抗肽、生理盐水,30d后全部处死,制作病理切片和HE染色。应用Olimpus CX41显微镜图像分析系统,计算在肿瘤组织的最大切面上,肿瘤坏死面积与肿瘤面积的比值。结果 Survivin拮抗肽实验组裸鼠的肿瘤坏死面积与肿瘤面积的比值大于对照组,差异具有统计学意义(P<0.05)。结论 Survivin拮抗肽对人胶质瘤U251细胞裸鼠移植瘤的生长具有抑制效应。
Objective To investigate the inhibiting effect of Survivin anti‐peptide on nude rats with brain glioma.Methods The rat orthotopic model of U251 glioma was established through transplanting human glioma U251 cells cultured in vitro in‐to caudate nucleus of nude rats by stereotactic technique. After 11 days ,all nude rats were randomly divided into experimental group and control group ,which were separately injected Survivin anti‐peptide and normal saline via abdomen injection. After 30 days ,we executed all of nude rats and made pathological sections and HE staining. Then the ratio of tumor necrotic area and tumor total area was calculated on the largest slice of tumor tissues by the analysis system of Olimpus CX41 microscope im‐age.Results The ratio of tumor necrotic area and tumor total area in experimental group was greater than that in control group （P〈0.05）.Conclusion The Survivin anti‐peptide have inhibitory effect on the development of tumors transplanted by human glioma U251 cells in nude rats.
出处
《中国实用神经疾病杂志》
2015年第18期1-2,共2页
Chinese Journal of Practical Nervous Diseases
基金
内蒙古自然科学基金(编号200408020902)
内蒙古医学院第一附属医院重大项目(编号200405)
