摘要
应用脑立体定向术将Ｃ＿６．细胞接种在Ｓｐｒｅｇｕｅ－Ｄａｗｌｅｙ大鼠脑尾状核，同时留置永久性聚乙烯导管建立的大鼠脑胶质瘤模型方法。将２０只ＳＤ大鼠按肿瘤生长时间分为４组。分别在接种后１、２、３、４周时进行ＭＲＩ检查，病理解剖，病理组织学以及ＧＦＡＰ和Ｓ－１００免疫组化检查。结果表明该方法建立胶质瘤模型不仅较为接近人脑胶质瘤生长特性，而且颅内生长稳定，无颅外种植生长，适于胶质瘤实验治疗研究需要。
The model of rat cerebral glioma estab-lished by stereotactic
inoculating C6 cells intoSpregue-Dawley rat caudate nucleus,followingpermanant implantation
of a polyvinylcatheter ,is discribed in this paper,MRI,pathoanatomy,histology and both GFAP
andS一100 protein immunohischemistry of gliomamodels were respectivily observed in 20
rats,which were divided into four groups at lst,2nd, 3rd and 4th week of
postinoculation,Theexperimental results demonstrated that tumorof models were not only
similar to humanglioma growing characters, but also stablegrowing at intracerebral and no
extracranialgrowth.It is can be better used in experimen-tal therapy of gliomas.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
1995年第4期221-222,共2页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金
