摘要
目的:研究桂枝挥发油及主要成分桂皮醛抗甲型流感病毒A/PR/8/34(H1N1)作用及其抗病毒作用机制。方法:KM小鼠70只随机分为7组,分别为正常组,模型组,利巴韦林组(0.15 g·kg-1),桂枝挥发油高、低剂量组(0.348,0.174mg·kg-1),桂皮醛高、低剂量组(0.264,0.132 mg·kg-1),每组10只。将浓度为30 LD50的H1N1病毒液50μL注入小鼠气管,复制病毒性肺炎模型小鼠,观察连续ig给药5 d对模型小鼠肺组织血凝滴度的抑制作用;ELISA法观察上述组别连续ig给药5 d对模型小鼠血清细胞因子白细胞介素-2(IL-2),IL-6和肿瘤坏死因子-α(TNF-α)分泌量的影响;流式细胞仪法测定桂枝挥发油0.348 mg·kg-1,桂皮醛0.264 mg·kg-1,正常组,模型组及利巴韦林组连续ig给药5 d对模型小鼠外周血T细胞亚群的影响。结果:与正常组比较,模型组小鼠肺组织血凝滴度,小鼠血清IL-6,TNF-α含量明显升高,小鼠外周血CD4+/CD8+,小鼠血清IL-2含量明显降低(P<0.05);与模型组比较,桂枝挥发油0.348 mg·kg-1,桂皮醛0.264 mg·kg-1能明显降低模型小鼠肺组织血凝滴度(P<0.05),桂皮醛0.264 mg·kg-1和桂枝挥发油0.348 mg·kg-1分别显著降低模型小鼠血清IL-6,TNF-α含量(P<0.05),桂皮醛0.264 mg·kg-1可使模型小鼠外周血CD4+/CD8+明显升高(P<0.05),各药物对小鼠血清IL-2含量的影响并不明显。结论:桂枝挥发油、桂皮醛治疗给药能明显降低模型小鼠肺组织血凝滴度,表现出较好的抗流感病毒作用。抗病毒机制与调控模型小鼠血清细胞因子IL-2,IL-6,TNF-α分泌水平及提高外周血T细胞亚群的比例有关。
Objective： To study the effect and anti-influenza A virus （H1 N1 ） mechanism of volatile oil from Cinnamomi Ramulus （VOCR） and its major component cinnamaldehyde in vivo. Method： Totally 70 KM mice were divided into seven groups： the control group, the model group, the Ribavirin group （0. 15 g·kg^-1）, VOCR high and low dose groups （0.348, 0. 174 mg·kg^-1） and cinnamaldehyde high and low dose groups （0.264, 0. 132 mg·kg^-1） , with 10 mice in each group. The model group was intratracheally injected with 50 μL H1N1 liquid to reproduce the H1 N1 model and then continuously given drugs for 5 d to observe the inhibitory effect in the hemagglutination titer interleukin-2 （ IL-2）, in lung tissues. The ELISA test was conducted to observe the effect on the secretions of serum IL-6 and tumor necrosis factor-α （TNF-α）. Flow cytometry was used to detect the effect on T cell subsets after the five-day continuous treatment in the VOCR （0. 348 mg·kg^-1） group, the cinnamaldehyde （0. 264 mg·kg^-1 ） group, the control group, the model group and the Ribavirin （0.15 g ·kg^-1 ） group. Result： Compared with the normal group, the model group showed significant increases in the hemagglutination titer in lung tissues, mice serum IL-6 and TNF-α but notable decreases in CD4^＋/CD8^＋ and IL-2 content （P 〈0.05）. Compared with the normal group, the VOCR （0. 348 mg ·kg^-1 ） group and the cinnamaldehyde （0. 264 mg ·kg^-1） group showed reduction in the serum levels of IL-6 and TNF-α separately （P 〈 0.05 ）; the Cinnamaldehyde （0.264 mg·kg^-1） obviously increased the rate of CD4^＋ to CD8^＋ T cells （P 〈 0.05）. All groups show no remarkable effect on serum IL-2 content. Conclusion： VOCR and cinnamaldehyde could significantly decrease the hemagglutination titer in lung tissues of H1N1-infected mice and show a better anti-H1 N1 effect. The mechanism of anti-influenza A virus effect s correlated with the regulation of the ratio between the secretions o
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第18期139-143,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(30973923)
“2010年度高层次留学人才回国资助人选”项目
“2010年度留学回国人员科技活动项目择优”项目(国家人社部人社厅函[2010]411,412号)
四川省教育厅培育项目(12ZB037)
四川省科技厅省青年科技创新研究团队专项计划项目(2014TD0007)
