摘要
目的观察黄芪甲苷和齐墩果酸对体外神经干细胞增殖和相关基因Jagged1表达的影响。方法采用机械吹打法从胎鼠脑内获得神经干细胞,随机分组,分别给予不同浓度(10-6,5×10-7,10-7,5×10-8,10-8,5×10-9,10-9 mol·L-1)的黄芪甲苷、齐墩果酸进行干预,72 h后用WST法检测细胞的增殖情况,PCR检测Jagged1表达水平。结果黄芪甲苷对体外神经干细胞增殖影响不显著,高剂量尚存在细胞毒性。齐墩果酸能促进神经干细胞增殖,且呈一定的剂量效应关系,与对照组和黄芪甲苷组比较均有显著差异(P<0.05)。细胞未分化状态下Jagged1基因存在一定的表达,增殖率越高Jagged1表达水平越高;与同浓度黄芪甲苷组比较,齐墩果酸显著上调Jagged1表达(P<0.05)。结论不同浓度的黄芪甲苷和齐墩果酸对体外培养神经干细胞增殖和Jagged1表达的影响不同;齐墩果酸作用优于黄芪甲苷。
OBJECTIVE To explore the effects of two effective components of traditional Chinese medicine（astragaloside A and oleanolic acid） on the proliferation of neural stem cells and the expression of Jagged1 mRNA in vitro. METHODS Neural stem cells were obtained from fetal mice＇s brain by mechanical degestion, and randomly divided, treated with different concentration（10^-6, 5×10^-7, 10^-7, 5×10^-8, 10^-8, 5×10^-9, 10^-9 mol·L^-1） of astragaloside A and oleanolic acid. Cell proliferation was checked by WST assay after 72 h intervention. Expression of Jagged1 mRNA was checked by PCR. RESULTS There was no significant effect of astragaloside A on the proliferation of neural stem cells. The high dose of astragaloside A had cytotoxic effect. Oleanolic acid promoted the proliferation of neural stem cells which showed dose-effect relationship, compared with control group and astragaloside A, there was significant difference（P〈0.05）. There was some expression of Jagged1 mRNA in undifferentiational neural stem cells, and its level was up-regulated according to the rate of proliferation. The expression of Jagged1 mRNA was significant different between astragaloside A and oleanolic acid（P〈0.05）. CONCLUSION There are different effects on proliferation of neural stem cell and expression of Jagged1 mRNA according to the concentration and the kinds of effective components of traditional Chinese medicine. The effect of oleanolic acid is better than astragaloside A.
出处
《中国现代应用药学》
CAS
CSCD
2015年第9期1033-1036,共4页
Chinese Journal of Modern Applied Pharmacy
基金
国家自然科学基金(30873355
81273989)
国家重点基础研究发展计划(2010CB530402)
