摘要
利用响应面法优化忽地笑多糖的水提工艺。在单因素实验的基础上,以提取温度、提取时间、液料比为变量,以忽地笑中多糖得率为响应值,应用Box-Benhnken中心组合实验建立多糖提取率的二次多项式回归方程,得出最佳提取工艺为:提取温度70℃、提取时间7.7 h、液料比23∶1(m L∶g),此条件下忽地笑多糖得率为42.00%。采取优化的提取工艺提取忽地笑、中国石蒜、稻草石蒜、玫瑰石蒜和石蒜中根、鳞茎和叶部位的多糖,并测定、比较其含量,实验结果显示,不同石蒜属植物中鳞茎部位多糖含量显著高于根与叶,其中石蒜鳞茎中多糖含量最高,达49.21%。
The extraction of pelysaecharide from the bulb of Lycoris aurea was investigated by response surface methodol- ogy. After optimized by single factor test, the extraction temperature, extraction duration and liquid/solid ratio were se- lected as the impact factor and the yield of polysaccharide was set as response value. The regression equation for extrac- tion yield of polysaccharide was established through Box-Benhnken central-united experiment design. The optimal extrac- tion condition for polysaecharide was determined as follows ： extraction temperature of 70 ~C, extraction duration of 7.7 h, liquid/solid ratio of 23：1 （mL： g）. Under the optimized extraction conditions,the yield of polysaeeharides was 42%. In addltion,the content of polysaccharide in bulb,stem and leaf of different species in genus Lycoris was further investi- gated. The results showed that the content of polysaccharide in bulbs was significantly higher than that in stem and leaf. The highest content of polysaeeharides was detected in the bulb of h radiata,which was up to 49.21%.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2015年第9期1576-1581,1606,共7页
Natural Product Research and Development
基金
湖南省科技厅科技计划(2013NK4107)
张家界市科技局计划(2014YB17)
吉首大学校级科研项目(14JD Y062)
湖南省研究生科研创新项目(CX2015B546)
关键词
忽地笑
多糖提取
响应面法
不同部位
Lycoris aurea
polysaccharides extraction
response surface methodology
different active part
