摘要
本研究旨在探讨黄芪多糖对免疫抑制模型小鼠CD4+CD25+调节性T细胞(Treg细胞)和CD4+Th17细胞亚群功能的影响。通过小鼠腹腔注射环磷酰胺建立免疫抑制动物模型,应用黄芪多糖对免疫抑制小鼠进行治疗,采用流式细胞仪分析脾脏中CD4+T细胞、CD4+CD25+Treg细胞的比例,ELISA检测血清IL-17的水平。结果表明:与模型对照组比较,黄芪多糖高、中剂量明显降低小鼠脾脏CD4+T细胞数的比例(P<0.05),同时黄芪多糖上调小鼠脾脏CD4+CD25+Treg细胞数的比例(P<0.05),降低免疫抑制小鼠血清IL-17的水平,尤其1.0 g/(kg·d)剂量组的IL-17水平下降较为明显(P<0.01)。提示黄芪多糖具有提高免疫抑制小鼠CD4+CD25+Treg细胞数的比例、降低CD4+T细胞的数量及抑制IL-17分泌活性的作用。
Astragalus membranaceus is a well-known traditional Chinese herbal medicine that has been used as a tonic herb in various immuno-deficient diseases. In order to investigate the effects of astragalus polysaccharides（APS） on CD4^＋CD25^＋regulatory T cells（CD4^＋CD25^＋Treg） and CD4^＋Th17 cells in immunesuppressed mice,the immunesuppressed mouse models were established by intraperitoneal injection with cyclophosphamide. After the treatments with APS,the proportion of CD4^＋T cells or CD4^＋CD25^＋Treg cells in spleen were analyzed by flow cytometry and the serum interleukin-17（IL-17） factor was tested by enzyme-linked immunosorbent assay（ELISA）. Compared with the model control mice,the proportion of CD4^＋T cells obviously decreased and the proportion of CD4^＋CD25^＋Treg cells significantly increased in mice of APS high-dose group and APS middle-dose group（P 0. 05）,and the level of serum IL-17 significantly reduced in mice of APS high-dose [1. 0 g /（kg·d） ] group（P 0. 01）. The results showed that APS can up-regulate the proportion of CD4^＋CD25^＋Treg cells and inhibit the cytokine secretion of IL-17 in immunesuppressed mice.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2015年第1期153-157,共5页
Natural Product Research and Development
基金
兰州大学"国家级大学生创新创业训练计划"项目(201310730157)
兰州大学口腔医学扶持基金项目(533000-432378)
