摘要
筛选产米渣蛋白水解酶菌株以促进米渣的绿色开发及充分利用。采用酪蛋白平板法从富含蛋白的样品初筛到115株产蛋白酶菌株。初筛菌株以米渣为唯一碳、氮源经发酵培养,通过复筛以分析其所产的蛋白酶,从豆豉中获得一株产米渣蛋白水解酶的目标菌株HP60。该菌株的粗酶活和对米渣蛋白的水解度分别达(263±7)U/m L和15.63%±0.30%。通过形态学、生理生化特征(API 20 NE,Biolog GP)及16S r RNA序列分析,鉴定HP60为解淀粉芽孢杆菌(Bacillus amyloliquefaciens),并已保藏于中国典型培养物保藏中心,保藏编号:CCTCC NO.M 2014200。本研究筛选的Bacillus amyloliquefaciens HP60对米渣蛋白的微生物开发奠定了一定基础。
Screening of protease-producing strains（PPS） was used for hydrolysis of rice dreg protein（RDP）,which could promote green development and make full use of RDP. A total of 115 PPS were isolated from samples protein-rich by casein plate method. These PPS were cultured with RDP as sole carbon and nitrogen source. Then,one target strain HP60 was obtained from fermented soybean by analysis of proteases of the fermentation broth via further screenings. Enzyme activity of HP60 and degree of hydrolysis of RDP were reached（263±7） U/m L and 15.63%±0.30%,respectively. The HP60 was identified as Bacillus amyloliquefaciens by morphological,physiological and biochemical characteristics（API 20 NE,Biolog GP） and 16 S r RNA sequence analysis and collected in China Center for Type Culture Collection（CCTCC）,collection number：CCTCC NO. M2014200. In this study,Bacillus amyloliquefaciens HP60 have laied a preliminary foundation for microbial utilization of rice dreg protein.
出处
《食品工业科技》
CAS
CSCD
北大核心
2015年第18期170-175,181,共7页
Science and Technology of Food Industry
基金
贵州省科学技术基金项目(黔科合J字[2010]2064号)
贵州省猪肉制品工程技术研究中心项目(黔科合农G字[2013]4001号)
关键词
米渣蛋白
产蛋白酶
筛选
鉴定
解淀粉芽孢杆菌
rice dreg protein
protease-producing
screening
identification
Bacillus amyloliquefaciens
