摘要
目的探讨肝癌患者重组人血管内皮抑制素联合肝动脉化疗栓塞后血清血管内皮生长因子(vascular endothelial growth factor,VEGF)、乏氧诱导因子-1α(hypoxia inducible factor-1 alpha,HIF-1α)、骨桥蛋白(osteopontin,OPN)及结缔组织生长因子(connective tissue growth factor,CTGF)的变化及临床意义。方法选取青海大学附属医院收治的中晚期原发性肝癌患者90例,随机分为对照组和观察组,每组各45例,对照组实施常规肝动脉化疗栓塞治疗,观察组实施重组人血管内皮抑制素注射液联合肝动脉化疗栓塞治疗;于治疗前、术后3、5 d,用酶联免疫吸附法动态监测分析血清中VEGF、HIF-1α、OPN、CTGF的水平,并分析相关性。结果对照组和观察组术后3、5 d与术前比较,VEGF、HIF-1α、OPN、CTGF水平均明显升高(P<0.05)。2组术后5 d与术后3 d比较,VEGF、HIF-1α、OPN、CTGF水平均显著下降(P<0.05)。观察组患者术后5 d除HIF-1α外,VEGF、OPN、CTGF水平均显著低于同期对照组(P<0.05)。90例肝癌患者外周血清中VEGF与HIF-1α、OPN、CTGF浓度水平显著正相关(r=0.985、0.995、0.959,P<0.05),HIF-1α与OPN、CTGF浓度水平高度正相关(r=0.842、0.874,P<0.05),OPN与CTGF浓度水平中度正相关(r=0.755,P<0.05)。结论重组人血管内皮抑制素联合肝动脉化疗栓塞治疗原发性肝癌的临床疗效明显,肝癌患者外周血清VEGF、HIF-1α、OPN及CTGF密切相关,可以反映临床疗效。
Objective To explore the effect of recombinant human endostatin combined with transcatheter arterial chemoembolization on vascular endothelial growth factor （VEGF）, hypoxia inducible factor-1 alpha（HIF-1α）, osteopontin （OPN） and connective tissue growth factor （CTGF） levels in serum of HCC patients.Methods 90 cases of primary liver cancer patients were randomly divided into control group and observation group, 45 cases in each group.The control group received the routine chemotherapy of hepatic artery embolization, and the observation group received recombinant human vascular endostatin combined with hepatic arterial chemoembolization.The serum levels of VEGF, HIF-1α, OPN, and CTGF were detected by enzyme-linked immunosorbent assay before treatment, 3 days and 5 days after operation, then the correlations were analyzed.Results The VEGF, HIF-1α, OPN, CTGF levels after 3 days and 5 days were higher than those before surgery in control group and observation group （P〈0.05）, the above indexes after 5 days were lower than those after 3 days in both groups （P〈0.05）, and VEGF, OPN and CTGF levels except for HIF-1αin observation group were significantly lower than those in control group after 5 days （P〈0.05）.There existed significant positive correlation between serum VEGF and HIF-1α, OPN, CTGF concentration levels（r=0.985, 0.995, 0.959,P〈0.05） in 90 cases of liver cancer patients, highly positive correlation between HIF-1αand OPN, CTGF levels （r=0.842,0.874, P〈0.05）, and moderately positive correlation between OPN and CTGF concentrations （r=0.755,P〈0.05）.Conclusion The efficacy of recombinant human vascular endostatin combined with transcatheter arterial chemotherapy and embolization in the treatment of primary hepatocellular carcinoma is good.There exsits closely correlation of VEGF, HIF-1α, OPN and CTGF levels in serum, which could reflect clinical efficacy.
出处
《中国生化药物杂志》
CAS
2015年第6期98-101,共4页
Chinese Journal of Biochemical Pharmaceutics
关键词
重组人血管内皮抑制素
肝癌
化疗栓塞
血管内皮生长因子
乏氧诱导因子-1Α
骨桥蛋白
结缔组织生长因子
recombinant human vascular endostatin
liver neoplasms
chemoembolization
vascular endothelial growth factor
hypoxia inducible factor-1 alpha
osteopontin
connective tissue growth factor
