摘要
目的 :探索并评价艾芬地尔对抑郁模型小鼠的作用。方法:采用强迫游泳试验及获得性无助试验,观察艾芬地尔对这两种抑郁动物模型行为学的影响。给药方式均为腹腔注射:低(1mg/kg)、中(2mg/kg)、高(3mg/kg)剂量试药组分别注射酒石酸艾芬地尔溶液1ml/只。空白对照组或模型对照组注射等体积生理盐水,阳性药物对照组注射等体积氯胺酮(1mg/kg)。(1)强迫游泳试验:150只小鼠随机分为3批,每批分为低、中、高剂量试药组、空白对照组、阳性药物对照组,每组10只。3批小鼠分别在给药后2h、4h、6h进行强迫游泳试验,记录小鼠累计不动时间。(2)获得性无助试验:小鼠经诱导造模后,随机选取其中80只造模成功的小鼠分为2批,每批分为低、高剂量试药组、阳性药物对照组、模型对照组,每组10只。另取20只未经造模的小鼠随机分为2组,分别作为以上2批中的空白对照组。2批小鼠分别在给药后2h和4h进行测试,记录小鼠逃避失败次数。结果:(1)强迫游泳试验:在给药2h及4h后艾芬地尔高、中、低剂量组与空白对照组比较小鼠的累计不动时间均有显著性差异(P<0.05),但各剂量组间无显著差异(P>0.05);在给药6h后,各剂量组与空白对照组比较累计不动时间均无显著性差异(P>0.05)。(2)获得性无助试验:模型对照组逃避失败次数显著高于空白对照组(P<0.01);在给药2h及4h后艾芬地尔低、高剂量组、阳性药物对照组与模型对照组相比小鼠的逃避失败次数的减少均有显著性差异(P<0.05),但各剂量组间均无显著性差异(P>0.05)。结论:在1mg/kg^3mg/kg的剂量范围内,腹腔注射酒石酸艾芬地尔能有效改善抑郁模型小鼠的行为,2h即可产生作用,药效可维持4h。
Objective： The antidepressant effect of ifenprodil on the mice model of depression will be explored and evaluated. Methods：Forced swimming test and learned helplessness test were selected to observe the effect of ifenprodil on the mice model of depression. Intraperitoneal injection is selected as the administration. The mice in the low（1mg/kg）, medium（2mg/kg） and high（3mg/kg） dose of the test groups were injected with 1ml of ifenprodil tartrate. The blank control groups or model control groups were injected with the same volume of saline, and the positive control groups were injected with the same volume of ketamine（1mg/kg）.（1）Forced swimming test. One hundred and fifty mice were divided randomly into three batches, each consisting of the low, medium, and high dose of test group, the blank control group and the positive control group（n=10）. The tests for each batch of mice were taken after 2 hours, 4 hours and 6 hours respectively and the immobility time of mice were recorded.（2） Learned helplessness test. In this test, mice were modeling firstly. Eighty mice those were successful modeling were divided randomly into two batches, each consisting of the low and high dose of test group, the positive control group, and the model control group（n=10）. Another 20 mice without modeling were divided randomly into two groups, as the blank control group（n=10） in each batch. The tests for each batch of mice were taken after 2 hours and 4 hours respectively and the times of fail to escape of mice were recorded. Results：（1） Forced swimming test. The cumulative immobility time of the high, medium, and low dose of test group was both less than the blank control group at each two hours and four hours after administration（P〉0.05）, and there is no significant difference among the three test groups（P〉0.05）. There is no significant difference among the three test groups and the blank control group at six hours after administration（P〉0.05）.（2）Learned helplessness test
出处
《西藏医药》
2015年第3期8-11,共4页
Tibetan Medicine
关键词
艾芬地尔
抗抑郁作用
强迫游泳
获得性无助
ifenprodil
antidepressant
forced swimming test
learned helplessness test
