摘要
目的 评价海马哺乳动物雷帕霉素靶蛋白（mTOR）信号通路在老龄大鼠脾切除术后认知功能障碍中的作用.方法 健康雄性SD大鼠63只,18月龄,体重400～ 540 g,采用随机数字表法,将其分为3组（n=21）：对照组（C组）、手术组（O组）和mTOR抑制剂雷帕霉素组（M组）.分别于术前、术后1、3和7d时进行Morris水迷宫实验测试认知功能.于术后1、3和7d水迷宫实验结束后每组处死7只大鼠,取海马组织,采用Western blot法测定mTOR和丝氨酸第396位点磷酸化tau（pS396 tau）蛋白的表达水平.结果 与C组相比,O组术后1和3d时逃避潜伏期延长,靶象限活动时间比率降低,海马pS396 tau蛋白表达上调,术后各时点海马mTOR表达上调,M组术后1和3d时逃避潜伏期延长,靶象限活动时间比率降低,海马mTOR和pS396 tau蛋白表达上调（P＜0.05）;与O组相比,M组术后1和3d时逃避潜伏期缩短,靶象限活动时间比率升高,海马pS396 tau蛋白表达下调,术后各时点海马mTOR表达下调（P＜0.05）.结论 海马mTOR信号通路参与了老龄大鼠脾切除术后认知功能障碍的发生.
Objective To evaluate the role of hippocampal mammalian target of rapamycin （mTOR） signaling pathways in the cognitive dysfunction after splenectomy in aged rats.Methods Sixtythree male Sprague-Dawley rats,aged 18 months,weighing 400-540 g,were randomly divided into 3 groups （n=21 each） using a random number table：control group （group C）,operation group （group O） and mTOR inhibitor rapamycin group （group M）.Morris water maze test was performed to evaluate cognitive function before operation and at 1,3 and 7 days after operation.After the end of Morris water maze test carried out at 1,3 and 7 days after operation,7 rats selected randomly in each group were sacrificed,and the brains were removed for detection of the expression of mTOR and phosphorylated tau protein at Ser-396 site （pS396 tau） in hippocampal tissues by using Western blot.Results Compared with group C,the escape latency was significantly prolonged,the ratio of time spending in the target quadrant was decreased,and the expression of pS396 tau was up-regulated at 1 and 3 days after operation,and the expression of mTOR was up-regulated at each time points after operation in group O,and the escape latency was significantly prolonged,the ratio of time spending in the target quadrant was decreased,and the expression of mTOR and pS396 tau was up-regulated at 1 and 3 days after operation in group M.Compared with group O,the escape latency was significantly shorten,the ratio of time spending in the target quadrant was increased,and the expression of pS96 tau was down-regulated at 1 and 3 days after operation,and the expression of mTOR was down-regulated at each time point after operation in group M.Conclusion The hippocampal mTOR signaling pathways are involved in the development of cognitive dysfunction after splenectomy in aged rats.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2015年第6期704-706,共3页
Chinese Journal of Anesthesiology
关键词
受体作用蛋白丝氨酸苏氨酸激酶类
海马
认知障碍
手术后并发症
老年人
Receptor-interacting protein serine-threonine kinases
Hippocampus
Cognition disorders
Postoperative complications
Aged
