摘要
目的探讨小鼠腹腔注射百草枯对其血清总抗氧化态的影响。方法小鼠随机分为对照组和百草枯染毒组(PQ组),PQ组小鼠又分别按照50、70、100、150、200和300 mg/kg的百草枯染毒剂量分成6组,给予一次性腹腔注射百草枯;PQ组小鼠分别在染毒后1 h和3 h留取血样,与对照组比较血清ABTS自由基清除率;每个血样采集点均有5个样本。结果不同染毒剂量组小鼠染毒后1 h、3 h的血清ABTS自由基清除率显著低于对照组小鼠(P<0.05);血清ABTS自由基清除率与染毒剂量和检测时间之间无显著相关性。结论百草枯染毒后小鼠的血清ABTS自由基清除率下降,血清总抗氧化态降低。
Objective To observe the change of serum total antioxidant status（ TAS） of mice treated with paraquat by injection ip. Methods The mice were randomly divided into control groups and PQ groups,and the mice in PQ groups were redivided into 6 subgroups,which were received 50、70、100、150、200 and 300 mg / kg,respectively,of paraquat by i. p.injection. The blood samples were taken at 1 h and 3 h after administration,respectively for measurement of ABTS [2, 2＇-Azinobis-（ 3-ethylbenz-thiazoline-6-sulphonate） ], a signal of radical scavenging activity, 5 mice for each blood sampling point.Results The serum ABTS level at either 1 h or 3 h after PQ administration were all significantly lowered than that in control group（ P〈0. 05）,and the decrease was also not dependent on dosage or time. Conclusion The serum free radical scavenging activity and total antioxidant status were reduced in mice after PQ administration,which was not correleted to administration time and dosage.
出处
《中国工业医学杂志》
CAS
2015年第4期256-258,共3页
Chinese Journal of Industrial Medicine
关键词
百草枯中毒
ABTS自由基清除力
总抗氧化态
小鼠
paraquat poisoning（PQ）
ABTS [2
2＇-Azinobis-（3-ethylbenzthiazoline-6-sulphonate） ]
total antioxidant status（TAS）
mice
